Oncotarget

Research Papers:

The interaction between NOLC1 and IAV NS1 protein promotes host cell apoptosis and reduces virus replication

Chunyu Zhu, Fangliang Zheng, Junfeng Zhu, Meichen Liu, Na Liu, Xue Li, Li Zhang, Zaidong Deng, Qi Zhao and Hongsheng Liu _

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Oncotarget. 2017; 8:94519-94527. https://doi.org/10.18632/oncotarget.21785

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Abstract

Chunyu Zhu1,*, Fangliang Zheng1,*, Junfeng Zhu1, Meichen Liu1, Na Liu1, Xue Li1, Li Zhang1, Zaidong Deng1, Qi Zhao2,3 and Hongsheng Liu1,2

1Key Laboratory of Animal Resource and Epidemic Disease Prevention, School of Life Science, Liaoning University, Shenyang 110036, China

2Research Center for Computer Simulating and Information Processing of Bio-Macromolecules of Liaoning, Shenyang 110036, China

3School of Mathematics, Liaoning University, Shenyang 110036, China

*The first two authors should be regarded as joint first authors

Correspondence to:

Hongsheng Liu, email: liuhongsheng@lnu.edu.cn

Keywords: IAV, NS1, NOLC1, protein-protein interaction, virus replication

Received: August 14, 2017     Accepted: September 23, 2017     Published: October 10, 2017

ABSTRACT

NS1 of the influenza virus plays an important role in the infection ability of the influenza virus. Our previous research found that NS1 protein interacts with the NOLC1 protein of host cells, however, the function of the interaction is unknown. In the present study, the role of the interaction between the two proteins in infection was further studied. Several analyses, including the use of a pull-down assay, Co-IP, western blot analysis, overexpression, RNAi, flow cytometry, etc., were used to demonstrate that the NS1 protein of H3N2 influenza virus interacts with host protein NOLC1 and reduces the quantity of NOLC1. The interaction also promotes apoptosis in A549 host cells, while the suppression of NOLC1 protein reduces the proliferation of the H3N2 virus. Based on these data, it was concluded that during the process of infection, NS1 protein interacts with NOLC1 protein, reducing the level of NOLC1, and that the interaction between the two proteins promotes apoptosis of host cells, thus reducing the proliferation of the virus. These findings provide new information on the biological function of the interaction between NS1 and NOLC1.


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