Oncotarget

Research Papers:

Effects of silencing Rab27a gene on biological characteristics and chemosensitivity of non-small cell lung cancer

Xia Li, Haiying Wang, Qinggan Ni, Zhiyuan Tang, Jun Ni, Liqin Xu, Hua Huang, Songshi Ni and Jian Feng _

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Oncotarget. 2017; 8:94481-94492. https://doi.org/10.18632/oncotarget.21782

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Abstract

Xia Li4,*, Haiying Wang1,*, Qinggan Ni5,*, Zhiyuan Tang1, Jun Ni2, Liqin Xu1, Hua Huang3, Songshi Ni1 and Jian Feng1

1Department of Respiratory, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China

2Department of Rehabilitation, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China

3Department of Pathology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China

4Department of Respiratory, Yancheng Third People’s Hospital, Yancheng 224002, Jiangsu, China

5Department of Central Laboratory, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China

*These authors have contributed equally to this work

Correspondence to:

Jian Feng, email: doctorfeng50@126.com

Keywords: non-small cell lung cancer, Rab27a, biological characteristics, proliferation, migration

Received: July 25, 2017     Accepted: September 08, 2017     Published: October 10, 2017

ABSTRACT

Rab27a, a member of the Rab protein family, can regulate the tumor microenvironment and promote the development of the tumor. Elevated expression of Rab27a is closely connected with many human cancers containing non-small cell lung cancer (NSCLC). But the role of Rab27a in non-small cell lung cancer and its possible mechanism is particularly unclear. In this research, we explored the effect of silencing Rab27a in vitro and in vivo, furnishing evidence that Rab27a could be a potential therapeutic target in NSCLC. Compared with corresponding control cells, silencing Rab27a had decreased ability of cell proliferation, migration and invasion in vitro and slower growth of xenograft tumors in mice. The expressions of apoptosis-associated proteins were induced with a reduction of anti-apoptotic protein in the NSCLC cells down-regulated Rab27a. Furthermore, Rab27a was associated with resistance to conventional chemotherapeutic agents. Our findings suggested that Rab27a might play a critical role in increasing chemosensitivity in NSCLC.


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