Knockdown of GTPBP4 inhibits cell growth and survival in human hepatocellular carcinoma and its prognostic significance
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Wen-Bin Liu1,2, Wei-Dong Jia1,2, Jin-Liang Ma1,2, Ge-Liang Xu1,2, Hang-Cheng Zhou3, Yan Peng3 and Wei Wang4
1Department of Hepatic Surgery, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, P.R. China
2Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, Hefei 230001, P.R. China
3Department of Pathology, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, P.R. China
4Department of Medical Oncology, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, P.R. China
Wei Wang, email: firstname.lastname@example.org
Keywords: GTPBP4, hepatocellular carcinoma, oncogene, prognosis, therapeutic target
Received: July 02, 2017 Accepted: September 08, 2017 Published: October 05, 2017
GTP-binding protein 4 (GTPBP4), as a novel member of GTPases involved in the synthesis of 60S subunit and maturation, is closely related to cell proliferation and growth. Till now, a small number of existing studies have found a contradictory dual role of GTPBP4 in cancer. Whether the expression level of GTPBP4 in hepatocellular carcinoma (HCC) is associated with the patients’ prognosis or its function and underlying molecular mechanisms still remains unclear. In the present study, the above issues were explored for the first time. Our results showed that GTPBP4 was overexpressed in HCC and knockdown of GTPBP4 delayed cell proliferation, impaired colony formation ability, induced cell cycle arrest in G2/M period and promoted apoptosis in HCC cell lines. Besides, in vivo xenograft nude mice model revealed that GTPBP4 knockdown could significantly suppress HCC tumorigenesis. Gene microarray and further pathway enrichment analyses indicated that ERBB signaling pathway was the most significantly changed one. More importantly, high GTPBP4 expression level significantly correlated to the poor prognosis of HCC patients. Taken together, all these findings suggest that GTPBP4 serves as an oncogene and plays a pivotal role in HCC development, which will be a potential therapeutic target or a biomarker for HCC.
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