The association of IL-17A polymorphisms with IL-17A serum levels and risk of ischemic stroke
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Hua-Tuo Huang1,*, Yu-Lan Lu1,*, Rong Wang1, Hai-Mei Qin1, Chun-Fang Wang1, Jun-Li Wang1, Yang Xiang1, Jing Guo3, Yan Lan3 and Ye-Sheng Wei1,2
1Department of Clinical Laboratory, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, Guangxi, China
2Youjiang Medical University for Nationalities, Baise 533000, Guangxi, China
3Department of Dermatology, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, Guangxi, China
*These authors have contributed equally to this work
Ye-Sheng Wei, email: [email protected]
Yan Lan, email: [email protected]
Keywords: IL-17A, genetic, promoter, polymorphisms, ischemic stroke
Received: May 17, 2017 Accepted: September 05, 2017 Published: October 05, 2017
The aim of our study was to investigate the association of interleukin-17A (IL-17A) polymorphisms with IL-17A serum levels and risk of ischemic stroke (IS) in a Chinese population. 392 IS patients and 443 controls were included in this study. The polymorphisms of IL-17A gene were determined by Snapshot SNP genotyping assay and DNA sequencing. Serum IL-17A levels were measured by enzyme-linked immunosorbent assay (ELISA). We found that the G allele, GA and GG genotypes, and GA/GG vs. AA model of rs2275913 polymorphism were associated with increased risk of IS even after adjusted by clinical characters such as age, gender and diabetes (G vs. A: OR=1.27, 95% CI, 1.05~1.54, P=0.014; GA vs. AA: OR=1.72, 95% CI, 1.05~2.81, P=0.032; GG vs. AA: OR=1.99, 95% CI, 1.08~3.67, P=0.028; GA/GG vs. AA: OR=1.78, 95% CI, 1.11~2.86, P=0.017). Serum IL-17A levels were increased in IS patients compared with controls (P<0.01). Individuals carrying rs2275913 GA or GG genotype present higher serum IL-17A levels compared with the rs2275913AA genotype in the IS group (P<0.01). In conclusion, this is the first study reporting the rs2275913 polymorphism as a risk factor for IS, which may be partly explained by influencing the levels of IL-17A cytokine.
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