IGF1 gene polymorphisms associated with diabetic retinopathy risk in Chinese Han population
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Jian Zhang1, Xiao Chen2, Like Zhang2 and Yi Peng3
1Department of Endocrinology, Xingtai People’s Hospital, Xingtai 054031, Hebei, China
2Department of Medical Optometry, Ophthalmology Hospital, Xingtai 054001, Hebei, China
3Department of Endocrinology, The First People’s Hospital of Zhangjiakou, Zhangjiakou 075000, Hebei, China
Xiao Chen, email: [email protected]
Keywords: IGF1, diabetic retinopathy, t2dm, haplotype, serum concentration
Received: March 04, 2017 Accepted: June 18, 2017 Published: September 28, 2017
Objective: This study aimed to explore the association of insulin-like growth factor 1 gene (IGF1) polymorphisms with diabetic retinopathy (DR) in a Chinese Han population.
Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used for genotyping. Genotype frequencies were compared by chi-square test. Odds ratio (OR) with 95% confidence interval (95%CI) was calculated to express the risk intensity of DR. Linkage disequilibrium between IGF1 polymorphisms was analyzed by Haploview. Serum IGF1 concentration was measured by enzyme-linked immunosorbent assays (ELISA) and assessed by student’s t test.
Results: AG genotype of rs6218 and TT genotype of rs35767 were significantly associated with the elevated risk of DR (rs6218: OR=1.77, P=0.04; rs35767: OR=2.32, P=0.03) and type II diabetes mellitus (T2DM) (rs6218: OR=1.92, P=0.00. rs35767: OR=2.29, P=0.02). Only T allele of rs35767 significantly increased the risk of DR (OR=1.45, P=0.04), however, rs6218 (OR=1.92, P=0.00), rs35767 (OR=0.02, P=0.02) and rs5742612 (OR=2.21, P=0.04) showed obvious association with T2DM. Haplotypes were only associated with T2DM, but not DR. Minor allele homozygote of rs35767 was obviously correlated with serum IGF1 level.
Conclusion: IGF1 rs6218 and rs35767 polymorphisms contribute to the risk of DR. IGF1 rs35767 polymorphism may participate in the regulation of serum IGF1 concentration in DR.
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