Oncotarget

Research Papers:

Transcriptomic pathway analysis of urokinase receptor silenced breast cancer cells: a microarray study

Pavan B. Narayanaswamy, Tapan K. Baral, Hermann Haller, Inna Dumler, Kshitish Acharya and Yulia Kiyan _

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Oncotarget. 2017; 8:101572-101590. https://doi.org/10.18632/oncotarget.21351

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Abstract

Pavan B. Narayanaswamy1, Tapan K. Baral2, Hermann Haller1, Inna Dumler1, Kshitish Acharya2,3 and Yulia Kiyan1

1Department of Nephrology, Hannover Medical School, Hannover, Germany

2Shodhaka Life Sciences Private Limited, Bengaluru, India

3Institute of Bioinformatics and Applied Biotechnology, Bengaluru, India

Correspondence to:

Yulia Kiyan, email: kiian.ioulia@mh-hannover.de

Keywords: urokinase receptor; breast cancer; pathway analysis; microarray

Received: January 04, 2017    Accepted: August 31, 2017    Published: September 28, 2017

ABSTRACT

Urokinase plasminogen activator receptor (PLAUR) has been implicated in a variety of physiological and pathological conditions. The multi-functionality of PLAUR is due to its capacity to interact with many co-receptors to regulate extracellular proteolysis and intracellular signaling. Recent reports are identifying novel functions of PLAUR which were not evident in the past; however, the molecular mechanisms of PLAUR signaling are not completely understood. Here, we have compared the transcriptomes of silencing control (sicon) and PLAUR silenced (PLAURsi) MDA-MB-231 breast cancer cells on treatment with radiation. We isolated RNA from the cells, synthesized cDNA and measured the gene expression changes by microarray. We identified 24 downregulated and 53 upregulated genes, which were significantly (P-value < 0.005) affected by PLAUR silencing. Our analysis revealed 415 canonical pathways and 743 causal disease networks affected on silencing PLAUR. Transcriptomic changes and predicted pathways supported and consolidated some of the earlier understanding in the context of PLAUR signaling; including our recent observations in DNA damage and repair process. In addition, we have identified several novel pathways where PLAUR is implicated.


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