Oncotarget

Research Papers:

Screening epitopes on systemic lupus erythematosus autoantigens with a peptide array

Lin Wang _, Chenjun Hao, Yongqiu Deng, Yanbo Liu, Shiliang Hu, Yangang Peng, Manna He, Jinhu Fu, Ming Liu, Jia Chen and Xiaoming Chen

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Oncotarget. 2017; 8:85559-85567. https://doi.org/10.18632/oncotarget.20994

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Abstract

Lin Wang1, Chenjun Hao2, Yongqiu Deng2, Yanbo Liu1, Shiliang Hu1, Yangang Peng1, Manna He1, Jinhu Fu1, Ming Liu1, Jia Chen1 and Xiaoming Chen1

1Department of Rheumatology, Shaoyang Central Hospital, 422000 Shaoyang, China

2Obstetrics and gynecology, Guangzhou Panyu Hexian Memorial Hospital, 511400 Guangzhou, China

Correspondence to:

Lin Wang, email: dear_wang356@163.com

Xiaoming Chen, email: chenxm_cn@163.com

Keywords: systemic lupus erythematosus, epitope, peptide array, autoantibody

Received: April 11, 2017     Accepted: August 28, 2017     Published: September 18, 2017

ABSTRACT

Systemic lupus erythematosus (SLE) is a common autoimmune disease. Many autoantibodies are closely associated with SLE. However, the specific epitopes recognized and bound by these autoantibodies are still unclear. This study screened the binding epitopes of SLE-related autoantibodies using a high-throughput screening method. Epitope prediction on 12 SLE-related autoantigens was performed using the Immune Epitope Database and Analysis Resource (IEDB) software. The predicted epitopes were synthesized into peptides and developed into a peptide array. Serum IgG from 50 SLE patients and 25 healthy controls was detected using the peptide array. The results were then validated using an enzyme-linked immunosorbent assay (ELISA). The diagnostic efficiency of each epitope was analyzed using a ROC curve. Seventy-three potential epitopes were screened for using the IEDB software after the epitopes on the 12 SLE-related autoantigens were analyzed. Peptide array screening revealed that the levels of the autoantibodies recognized and bound by 4 peptide antigens were significantly upregulated in the serum of SLE patients (P < 0.05). The ELISA results showed that the 4 antigens with significantly increased serum autoantibodies levels in SLE patients were acidic ribosomal phosphoprotein (P0)-4, acidic ribosomal phosphoprotein (P0)-11, DNA topoisomerase 1 (full length)-1, and U1-SnRNP 68/70 KDa-1 (P < 0.05), and the areas under the ROC curve for diagnosing SLE on the basis of these peptides were 0.91, 0.90, 0.93, and 0.91, respectively. Many autoantibodies specifically expressed in the serum of patients with SLE can be detected by specific peptide fragments and may be used as markers in clinical auxiliary diagnoses.


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