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Improvement of therapeutic effects of mesenchymal stem cells in myocardial infarction through genetic suppression of microRNA-142

Liu-Xue Yang, Chun-Ling Wei, Man-Li Guo, Yong Zhang, Feng Bai and Shao-Gang Ma _

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Oncotarget. 2017; 8:85549-85558. https://doi.org/10.18632/oncotarget.20935

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Abstract

Liu-Xue Yang1,*, Chun-Ling Wei2,*, Man-Li Guo3, Yong Zhang2, Feng Bai2 and Shao-Gang Ma2

1Department of Endocrinology and Metabolism, The Second Hospital Affiliated to Guilin Medical College, Guilin 541100, China

2Department of Endocrinology and Metabolism, Huai'an Hospital Affiliated to Xuzhou Medical College, Huai'an Second People’s Hospital, Huai'an 223002, China

3Department of Endocrinology and Metabolism, Suqian People’s Hospital, Nanjing Drum Tower Hospital, Suqian 223800, China

*These authors contributed equally to this work

Correspondence to:

Shao-Gang Ma, email: msgylx@yeah.net

Keywords: mesenchymal stem cells (MSCs), microRNA-142, miR-142-depleted mesenchymal stem cells (md-MSCs), CXCR7, myocardial infarction (MI)

Received: June 20, 2017     Accepted: August 08, 2017     Published: September 15, 2017

ABSTRACT

Transplanted mesenchymal stem cells (MSCs) have been shown to contribute to myocardial repair after myocardial infarction (MI), primarily through production and secretion some growth factors and cytokines related to cell survival and regeneration. Further improvement of the therapeutic potential of MSCs appears to be an attractive strategy for MI treatment. CXC chemokine receptor (CXCR) 7 is the receptor for stromal cell-derived factor-1 (SDF-1), an important chemokine that is essential for tissue repair and angiogenesis. SDF-1/CXCR7 axis plays a critical role in the mobilization, recruitment and function of MSCs during tissue regeneration. Here, we depleted miR-142 that targets CXCR7 in MSCs cells through expression of antisense of miR-142, resulting in enhanced expression of CXCR7 in these miR-142-depleted MSCs (md-MSCs). In vitro, presence of md-MSCs reduced hypoxia-induced cardiac muscle cell apoptosis in a more pronounced manner than MSCs. In vivo, compared to transplantation of MSCs, transplantation of md-MSCs further enhanced cardiac re-vascularization and further improved cardiac functions after MI in mice. Together, our data suggest that depletion of miR-142 in MSCs may improve their therapeutic effects on MI.


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