Improvement of therapeutic effects of mesenchymal stem cells in myocardial infarction through genetic suppression of microRNA-142
Metrics: PDF 1585 views | HTML 1975 views | ?
Liu-Xue Yang1,*, Chun-Ling Wei2,*, Man-Li Guo3, Yong Zhang2, Feng Bai2 and Shao-Gang Ma2
1Department of Endocrinology and Metabolism, The Second Hospital Affiliated to Guilin Medical College, Guilin 541100, China
2Department of Endocrinology and Metabolism, Huai'an Hospital Affiliated to Xuzhou Medical College, Huai'an Second People’s Hospital, Huai'an 223002, China
3Department of Endocrinology and Metabolism, Suqian People’s Hospital, Nanjing Drum Tower Hospital, Suqian 223800, China
*These authors contributed equally to this work
Shao-Gang Ma, email: firstname.lastname@example.org
Keywords: mesenchymal stem cells (MSCs), microRNA-142, miR-142-depleted mesenchymal stem cells (md-MSCs), CXCR7, myocardial infarction (MI)
Received: June 20, 2017 Accepted: August 08, 2017 Published: September 15, 2017
Transplanted mesenchymal stem cells (MSCs) have been shown to contribute to myocardial repair after myocardial infarction (MI), primarily through production and secretion some growth factors and cytokines related to cell survival and regeneration. Further improvement of the therapeutic potential of MSCs appears to be an attractive strategy for MI treatment. CXC chemokine receptor (CXCR) 7 is the receptor for stromal cell-derived factor-1 (SDF-1), an important chemokine that is essential for tissue repair and angiogenesis. SDF-1/CXCR7 axis plays a critical role in the mobilization, recruitment and function of MSCs during tissue regeneration. Here, we depleted miR-142 that targets CXCR7 in MSCs cells through expression of antisense of miR-142, resulting in enhanced expression of CXCR7 in these miR-142-depleted MSCs (md-MSCs). In vitro, presence of md-MSCs reduced hypoxia-induced cardiac muscle cell apoptosis in a more pronounced manner than MSCs. In vivo, compared to transplantation of MSCs, transplantation of md-MSCs further enhanced cardiac re-vascularization and further improved cardiac functions after MI in mice. Together, our data suggest that depletion of miR-142 in MSCs may improve their therapeutic effects on MI.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.