Research Papers:

Maternal dietary manganese protects chick embryos against maternal heat stress via epigenetic-activated antioxidant and anti-apoptotic abilities

Yongwen Zhu, Lin Lu, Xiudong Liao, Wenxiang Li, Liyang Zhang, Cheng Ji, Xi Lin, Hsiao-Ching Liu, Jack Odle and Xugang Luo _

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Oncotarget. 2017; 8:89665-89680. https://doi.org/10.18632/oncotarget.20804

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Yongwen Zhu1,2,3,*, Lin Lu1,*, Xiudong Liao1, Wenxiang Li3, Liyang Zhang1, Cheng Ji3, Xi Lin4, Hsiao-Ching Liu4, Jack Odle4 and Xugang Luo1

1Mineral Nutrition Research Division, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

2College of Animal Science, South China Agricultural University, Guangzhou 510000, China

3College of Animal Science and Technology, China Agricultural University, Beijing 100193, China

4Department of Animal Science, North Carolina State University, Raleigh, NC 27695, USA

*These authors contributed equally to the present study

Correspondence to:

Xugang Luo, email: [email protected]

Keywords: epigenetics, maternal environmental hyperthermia, manganese superoxide dismutase, apoptosis, chick embryo

Received: July 12, 2017     Accepted: August 17, 2017     Published: September 11, 2017


Maternal heat stress induced the aberrant epigenetic patterns resulting in the abnormal development of offspring embryos. It is unclear whether maternal dietary manganese supplementation as an epigenetic modifier could protect the chick embryonic development against maternal heat stress via epigenetic mechanisms. To test this hypothesis using an avian model, a completely randomized design with a 2 (maternal normal and high environmental temperatures of 21 and 32°C, respectively) × 3 (maternal dietary manganese sources, the control diet without manganese supplementation and the control diet + 120 mg/kg as either inorganic or organic manganese) factorial arrangement was adopted. Maternal environmental hyperthermia increased mRNA expressions of heat shock proteins 90 and 70, cyclin-dependent kinase 6 and B-cell CLL/lymphoma 2-associated X protein displaying oxidative damage and apoptosis in the embryonic heart. Maternal environmental hyperthermia impaired the embryonic development associated with the alteration of epigenetic status, as evidenced by global DNA hypomethylation and histone 3 lysine 9 hypoacetylation in the embryonic heart. Maternal dietary manganese supplementation increased the heart anti-apoptotic gene B-cell CLL/lymphoma 2 expressions under maternal environmental hyperthermia and manganese superoxide dismutase enzyme activity in the embryonic heart. Maternal dietary organic Mn supplementation effectively eliminated the impairment of maternal environmental hyperthermia on the embryonic development. Maternal dietary manganese supplementation up-regulated manganese superoxide dismutase mRNA expression by reducing DNA methylation and increasing histone 3 lysine 9 acetylation of its promoter. It is suggested that maternal dietary manganese addition could protect the chick embryonic development against maternal heat stress via enhancing epigenetic-activated antioxidant and anti-apoptotic abilities.

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