Oncotarget

Research Papers:

The E2F activators control multiple mitotic regulators and maintain genomic integrity through Sgo1 and BubR1

Miyoung Lee, Yainyrette Rivera-Rivera, Carlos S. Moreno and Harold I. Saavedra _

PDF  |  HTML  |  How to cite

Oncotarget. 2017; 8:77649-77672. https://doi.org/10.18632/oncotarget.20765

Metrics: PDF 2094 views  |   HTML 9574 views  |   ?  


Abstract

Miyoung Lee1, Yainyrette Rivera-Rivera3, Carlos S. Moreno2 and Harold I. Saavedra3

1Aflac Cancer and Blood Disorders Center, Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia 30322, USA

2Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA

3Department of Basic Sciences, Program of Pharmacology, Ponce Health Sciences University-School of Medicine/Ponce Research Institute, Ponce, 00716-2348 Puerto Rico

Correspondence to:

Harold I. Saavedra, email: [email protected]

Keywords: centrosome, transcription factors, chromosome instability, breast cancer, mammary epithelial cells

Received: April 14, 2016    Accepted: August 14, 2017    Published: September 08, 2017

ABSTRACT

The E2F1, E2F2, and E2F3a transcriptional activators control proliferation. However, how the E2F activators regulate mitosis to maintain genomic integrity is unclear. Centrosome amplification (CA) and unregulated spindle assembly checkpoint (SAC) are major generators of aneuploidy and chromosome instability (CIN) in cancer. Previously, we showed that overexpression of single E2F activators induced CA and CIN in mammary epithelial cells, and here we show that combined overexpression of E2F activators did not enhance CA. Instead, the E2F activators elevated expression of multiple mitotic regulators, including Sgo1, Nek2, Hec1, BubR1, and Mps1/TTK. cBioPortal analyses of the TCGA database showed that E2F overexpression in lobular invasive breast tumors correlates with overexpression of multiple regulators of chromosome segregation, centrosome homeostasis, and the SAC. Kaplan-Meier plots identified correlations between individual or combined overexpression of E2F1, E2F3a, Mps1/TTK, Nek2, BubR1, or Hec1 and poor overall and relapse-free survival of breast cancer patients. In MCF10A normal mammary epithelial cells co-overexpressing E2Fs, transient Sgo1 knockdown induced CA, high percentages of premature sister chromatid separation, chromosome losses, increased apoptosis, and decreased cell clonogenicity. BubR1 silencing resulted in chromosome losses without CA, demonstrating that Sgo1 and BubR1 maintain genomic integrity through two distinct mechanisms. Our results suggest that deregulated activation of the E2Fs in mammary epithelial cells is counteracted by activation of a Sgo1-dependent mitotic checkpoint.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 20765