Blockade of potassium ion transports enhances hypotonicity-induced cytocidal effects in gastric cancer
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Toshiyuki Kosuga1, Atsushi Shiozaki1, Michihiro Kudou1, Yuzo Yamazato1, Daisuke Ichikawa1, Shuhei Komatsu1, Hirotaka Konishi1, Kazuma Okamoto1, Katsutoshi Shoda1, Tomohiro Arita1, Ryo Morimura1, Yasutoshi Murayama1, Yoshiaki Kuriu1, Hisashi Ikoma1, Masayoshi Nakanishi1, Hitoshi Fujiwara1, Yoshinori Marunaka2,3 and Eigo Otsuji1
1Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan
2Department of Molecular Cell Physiology and Bio-Ionomics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan
3Japan Institute for Food Education and Health, St. Agnes’ University, Kyoto, Japan
Atsushi Shiozaki, email: email@example.com
Keywords: gastric cancer; peritoneal lavage; peritoneal dissemination; potassium channel; regulatory volume decrease
Received: January 17, 2017 Accepted: August 07, 2017 Published: September 08, 2017
Background: Peritoneal lavage with distilled water has been used for surgeries of various cancers to reduce peritoneal recurrence. This study examined whether blockade of potassium ion transports enhances hypotonicity-induced cytocidal effects in gastric cancer (GC).
Results: A potassium channel blocker inhibited the occurrence of regulatory volume decrease (RVD) induced by mild hypotonic stimulation, and significantly enhanced cytocidal effects on GC cells. Incubating MKN45 cells with hypotonic solutions containing a potassium channel blocker significantly reduced the formation of peritoneal metastases in nude mice.
Methods: The three human GC cell lines (HGC-27, Kato III, and MKN45) were exposed to mild hypotonic solutions, and the effects of blockade of potassium ion transports during hypotonic stimulation on cell volume changes and cell viabilities were examined. In the in vivo study, MKN45 cells stimulated with mild hypotonic solutions were intraperitoneally injected into nude mice, and the effects of blockade of potassium ion transports during hypotonic stimulation on the formation of peritoneal metastases were evaluated.
Conclusions: Blockade of potassium ion transports enhances hypotonicity-induced cytocidal effects on GC cells, which may contribute to development of a novel lavage method for further reduction of peritoneal recurrence in GC.
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