Aflatoxin B1 affects apoptosis and expression of death receptor and endoplasmic reticulum molecules in chicken spleen
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Panpan Zhu1,2,*, Zhicai Zuo1,2,*, Zhixiang Zheng1,2,*, Fengyuan Wang1,2,*, Xi Peng3, Jing Fang1,2, Hengmin Cui1,2, Caixia Gao1,2, Hetao Song1,2, Yi Zhou4 and Xici Liu2
1Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China
2College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China
3College of Life Sciences, China West Normal University, Nanchong, Sichuan 637002, PR China
4Life Science Department, Sichuan Agricultural University, Yaan, Sichuan 625014, PR China
*These authors contributed equally to this work
Jing Fang, email: [email protected]
Xi Peng, email: [email protected]
Keywords: AFB1, splenocyte apoptosis, death receptor molecules, endoplasmic reticulum molecules, chicken
Received: May 27, 2017 Accepted: July 26, 2017 Published: August 30, 2017
Aflatoxin B1 (AFB1) is a natural product of the Aspergillus genus of molds, which grow on several foodstuffs stored in hot moist conditions, and is among the most potent hepatocarcinogens and immunosuppression presently known. The latter was related to the up-regulated apoptosis of immune organs. However, the effect of expression of death receptor and endoplasmic reticulum molecules in AFB1-induced apoptosis of chicken splenocytes was largely unknown. The objective of this study was to investigate this unknown field. One hundred and forty four one-day-old chickens were randomly divided into control group (0 mg/kg AFB1) and AFB1group (0.6 mg/kg AFB1), respectively and fed with AFB1 for 21 days. Histological observation demonstrated that AFB1 caused slight congestion and lymphocytic depletion in the spleen. TUNEL and flow cytometry assays showed the excessive apoptosis of splenocytes provoked by AFB1. Moreover, quantitative real-time PCR analysis revealed that AFB1 induced the elevated mRNA expression of Fas, FasL, TNF-α, TNF-R1, Caspase-3, Caspase-8, Caspase-10, Grp78 and Grp94 in the spleen. These findings suggested that AFB1 could lead the excessive apoptosis and alter the expression of death receptor and endoplasmic reticulum molecules in chicken spleen.
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