Oncotarget

Research Papers:

miR-137 acts as a tumor suppressor via inhibiting CXCL12 in human glioblastoma

Dehua Li, Wei Shan, Yan Fang, Pan Wang and Jicheng Li _

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Oncotarget. 2017; 8:101262-101270. https://doi.org/10.18632/oncotarget.20589

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Abstract

Dehua Li1,2,3, Wei Shan3, Yan Fang3, Pan Wang3 and Jicheng Li1,2

1Institute of Cell Biology, Zhejiang University, Hangzhou, 310031, China

2Department of Human Anatomy, Histology and Embryology, School of Medicine, Zhejiang University, Hangzhou, 310031, China

3Department of Anatomy, College of Basic Medical Sciences, Jinzhou Medical University, Jinzhou, 121000, China

Correspondence to:

Jicheng Li, email: leejczhejiang@163.com

Keywords: miR-137; CXCL12; GBM; suppressor

Received: June 21, 2017    Accepted: August 08, 2017    Published: August 24, 2017

ABSTRACT

Up to date, miR-137 has been demonstrated as a tumor suppressor in many kinds of human malignancies. In the present study, we conducted transfection, western blot and RT-PCR to explore the role of miR-137 in the development of human glioblastoma (GBM). Here, we found that miR-137 expression was obviously down-regulated in GBM tissues and cells rather than matched non-tumor tissues and NHA cells. However, the expression of C-X-C motif ligand 12 (CXCL12) mRNA and protein were up-regulated in GBM tissues and cells. In vitro, miR-137 mimics inhibited GBM cell proliferation, migration and invasion, and the 3′-untranslated regions (3′-UTR) of CXCL12 were a direct target of miR-137. In addition, miR-137 mimics also inhibited the expression of EGFR, Bcl-2 and MMP2/9 proteins, but increased the expression of Bax protein. Notably, CXCL12 over-expression attenuated miR-137-inhibited cell proliferation and invasion, while CXCL12 siRNAs promoted miR-137 inhibition effects. In vivo, miR-137 mimics also suppressed tumor growth in nude mice xenograft model. In conclusion, miR-137 serves as a tumor suppressor by inhibition of CXCL12 in human GBM. Thus, miR-137-CXCL12 can be recommended as a useful and effective target for treatment of GBM.


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