Research Papers:
The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma
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Abstract
Can Lu1, QiuYan He1, Wei Zhu1, ChunYan Fu1, JianHua Zhou1, YongGuang Tao2,3,4, Shuang Liu5 and DeSheng Xiao6
1Department of Pathology, Xiangya Hospital/ School of Basic Medicine, Central South University, Changsha, Hunan 410078, China
2Cancer Research Institute, School of Basic Medicine, Central South University, Changsha, Hunan 410078, China
3Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Hunan 410078, China
4Key Laboratory of Carcinogenesis, National Health and Family Planning Commission, Hunan 410078, China
5Center for Medicine Research, Xiangya Hospital, Central South University, Changsha, Hunan 410078, China
6Department of Pathology, Xiangya Hospital, School of Basic Medicine, Central South University, Changsha, Hunan 410078, China
Correspondence to:
Desheng Xiao, email: [email protected]
Keywords: immunoglobulin, gene rearrangement, B-cell lymphoma
Received: March 16, 2017 Accepted: June 29, 2017 Published: August 18, 2017
ABSTRACT
Objective: To discuss the clinical value of immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma.
Methods: A total of 209 cases of B-cell lymphomas and 35 cases of reactive lymphoid hyperplasia were selected for DNA extraction and PCR amplification using the BIOMED-2 primer system. Gel electrophoresis of heteroduplexes was used to analyze immunoglobulin gene rearrangements.
Results: A total of 209 cases of B-cell lymphoma, including 69 extranodal marginal zone B-cell lymphomas of mucosa-associated lymphoid tissue, 63 diffuse large B-cell lymphomas, 39 follicular lymphomas, 15 small lymphocytic lymphomas, 6 plasmacytomas, 6 mantle cell lymphomas, 7 nodal marginal zone B-cell lymphomas, and 4 lymphoplasmacytoid lymphomas, were examined. Immunoglobulin gene rearrangements were found in all 209 cases, with 93 IGHA, 122 IGHB, 98 IGHC, 167 IGK, 100 IGL, 167 IGHA/B/C, 204 IGH/IGK, 209 IGH/IGK/IGL, 129 IGH+IGK, 81 IGH+IGL, 83 IGK+IGL and 68 IGH+IGK+IGL gene rearrangements. Immunoglobulin gene rearrangements were not found in the 35 cases of reactive lymphoid hyperplasia. IGH and IGK gene rearrangements were mainly found in mantle cell lymphomas, small lymphocytic lymphomas, extranodal marginal zone B-cell lymphomas of mucosa-associated lymphoid tissue and diffuse large B-cell lymphomas. The IGH gene rearrangement was mainly found in lymphoplasmacytoid lymphomas and follicular lymphomas. IGK and IGL gene rearrangements were mainly found in plasmocytoma, and the IGK gene rearrangement was mainly found in nodal marginal zone B-cell lymphomas.
Conclusions: The BIOMED-2 standardized immunoglobulin gene rearrangement detection system is an important tool in B-cell lymphoma diagnosis. Analysis of IGH, IGK and IGL gene rearrangements is valuable in confirming the classification of B-cell NHL.
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