Research Papers:

Inhibiting PHD2 in bone marrow mesenchymal stem cells via lentiviral vector-mediated RNA interference facilitates the repair of periodontal tissue defects in SD rats

Changxing Chen, Houxuan Li, Jun Jiang, Qian Zhang and Fuhua Yan _

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Oncotarget. 2017; 8:72676-72699. https://doi.org/10.18632/oncotarget.20243

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Changxing Chen1,2, Houxuan Li1, Jun Jiang3, Qian Zhang1,2 and Fuhua Yan1,2

1Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, 210008, China

2Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, 210008, China

3Stomatological Hospital of Fujian Medical University, Fuzhou, Fujian, 350001, China

Correspondence to:

Fuhua Yan, email: [email protected]

Keywords: lentiviral vector, PHD2, stem cells, periodontal tissue defects, oxidative stress

Received: January 10, 2017     Accepted: July 25, 2017     Published: August 14, 2017


Hypoxia-inducible factors (HIFs) play an important role in angiogenesis, and they can activate the expression of several downstream angiogenic factors. HIF-1 is a major transcriptor of HIFs, composed of α and β subunits. Prolyl hydroxylase domain-containing protein 2 (PHD2) is the main catabolic enzyme for HIF-1α, and it can accelerate its degradation under normoxic conditions. PHD2 expression in bone marrow mesenchymal stem cells (BMMSCs) of SD rats was down-regulated under normoxic conditions in this study by utilizing lentiviral vector-mediated RNA interference to promote HIF-1α accumulation, thus enhancing the expression of angiogenic factors. A tissue-engineered compound was constructed using the composite collagen membrane of BMMSCs after PHD2 gene silencing to repair periodontal fenestration defects in SD rats. The results of this study indicated that, after PHD2 gene silencing, the osteogenic differentiation of BMMSCs was enhanced in vitro, the resistance of cells to oxidative stress was also validated in vitro, thereby illustrating the promotion of the repair of artificially constructed periodontal tissue defects in rats. The results of this study provide a reference and guidance for future applications of RNA interference in periodontal tissue engineering and serve as a basis for improving the survival of seed cells in recipient tissues.

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