Oncotarget

Research Papers:

MiR-196a2 and lung cancer in Chinese non-smoking females: a genetic association study and expression analysis

Zhihua Yin, Zhigang Cui, Yangwu Ren, Lingzi Xia, Hang Li and Baosen Zhou _

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Oncotarget. 2017; 8:70890-70898. https://doi.org/10.18632/oncotarget.20174

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Abstract

Zhihua Yin1,2, Zhigang Cui3, Yangwu Ren1,2, Lingzi Xia1,2, Hang Li1,2 and Baosen Zhou1,2

1Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110122, PR China

2Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang 110122, PR China

3School of Nursing, China Medical University, Shenyang 110122, China

Correspondence to:

Baosen Zhou, email: [email protected]

Keywords: lung cancer, microRNA, single nucleotide polymorphism, genetic susceptibility, expression

Received: January 05, 2017    Accepted: July 24, 2017    Published: August 10, 2017

ABSTRACT

Background: The common polymorphism rs11614913 in miR-196a2 might be associated with lung cancer risk for non-smoking females in northeast China.

Methods: The genotypes of rs11614913 in miR-196a2 were determined by a case-control study including 1003 patients with lung cancer and 1003 healthy controls. The tissues were detected to assess the miRNA expression. Secondary structures of miR-196a2 were predicted.

Results: There was a significant association between miR-196a2 rs11614913 and lung cancer risk in Chinese non-smoking females. Individuals carrying TC or CC genotype had increased risk of lung cancer compared with TT genotype (adjusted risks were 1.63 and 1.67). The C allele was associated with a higher risk of lung cancer with a significant risk of 1.27. The similar significant results were also found in lung adenocarcinoma. There was a significant association between miR-196a2 expression and lung cancer risk (t=2.594, P=0.012). The relative expression of miR-196a2 was significantly higher for CC genotype comparing with the CT or TT genotype in tumor tissues (P values were all 0.003). The optimal free energies were different for T allele and C allele.

Conclusions: The polymorphism rs11614913 in miR-196a2 may be associated with lung cancer risks in Chinese non-smoking females through affecting miR-196a2 expression and secondary structure.


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