Oncotarget

Research Papers:

miR-21 modulates the effect of EZH2 on the biological behavior of human lung cancer stem cells in vitro

Hui Xia _, Wen Zhang, Baoshi Zhang, Yingnan Zhao, Yunlong Zhao, Shaojun Li and Yang Liu

PDF  |  HTML  |  Supplementary Files  |  How to cite

Oncotarget. 2017; 8:85442-85451. https://doi.org/10.18632/oncotarget.20006

Metrics: PDF 1785 views  |   HTML 2591 views  |   ?  


Abstract

Hui Xia1,2,3, Wen Zhang1, Baoshi Zhang1, Yingnan Zhao1, Yunlong Zhao1, Shaojun Li1 and Yang Liu2

1Department of Thoracic-Cardio Surgery, First Affiliated Hospital of PLA General Hospital, Beijing, China

2Department of Thoracic Surgery, PLA General Hospital, Beijing, China

3Medical School of PLA, Beijing, China

Correspondence to:

Hui Xia, email: [email protected]

Yang Liu, email: China [email protected]

Keywords: miR-21, EZH2, lung cancer stem cells, radiotherapy, chemotherapy

Received: November 10, 2016    Accepted: June 19, 2017    Published: August 07, 2017

ABSTRACT

Non-small-cell lung cancer has a high mortality rate and poor prognosis. Therefore, novel therapeutic approaches are urgently needed to enhance patient survival rates. In this study, we investigated the effects of miR-21 and EZH2 on the biological behavior of human lung cancer stem cells in vitro. We found increased expression of EZH2 and miR-21 in LCSCs, and miR-21 overexpression increased EZH2 levels in LCSCs. In addition, EZH2 and miR-21 knockdown increased the sensitivity of LCSCs to chemo- and radiation therapy, and exogenous EZH2 expression rescued the effects of anti-miR-21. Cell proliferation was reduced by 39.2% and 69.7% in the presence of radio- or chemotherapy combined with anti-miR-21 transfection, respectively. The downstream molecules included Cdc2, cyclin B1, and Bcl-2, which are involved in the regulation of cell cycle and apoptosis and which could themselves be reduced or enhanced by changes in miR-21 and EZH2 levels in LCSCs. This study demonstrates the direct relationship between miR-21 and EZH2 which was increased by 43% after the application of the miR-21 mimic. Above data indicates that these two molecules can influence the biological behavior of LCSCs by altering their corresponding targets. Our findings support the potential roles of miR-21 and EZH2 in improving the therapeutic efficacy of clinical lung cancer treatments.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 20006