Oncotarget

Research Papers:

Circular RNAs promote TRPM3 expression by inhibiting hsa-miR-130a-3p in coronary artery disease patients

Ren-You Pan, Ping Liu, Hai-Tang Zhou, Wei-Xin Sun, Jun Song, Jiang Shu, Guo-Jing Cui, Zhi-Jian Yang and En-Zhi Jia _

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Oncotarget. 2017; 8:60280-60290. https://doi.org/10.18632/oncotarget.19941

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Abstract

Ren-You Pan1,*, Ping Liu1, Hai-Tang Zhou1, Wei-Xin Sun1, Jun Song1,*, Jiang Shu1, Guo-Jing Cui1, Zhi-Jian Yang2 and En-Zhi Jia2

1Department of Cardiovascular Medicine, Yancheng TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Yancheng 224000, Jiangsu Province, China

2Department of Cardiovascular Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China

*These authors contributed equally to this work

Correspondence to:

En-Zhi Jia, email: [email protected]

Keywords: microRNA, coronary heart disease, circRNA, ceRNA

Received: May 04, 2017     Accepted: July 25, 2017     Published: August 04, 2017

ABSTRACT

We investigated the differential expression of circular RNAs (circRNAs) in plasma samples from three coronary artery disease (CAD) patients to identify putative therapeutic targets. We identified 24 differentially expressed circRNAs (18 up-regulated and 6 down-regulated) and 7 differentially expressed mRNAs (6 up-regulated and 1 down-regulated) in CAD patients based on competing endogenous RNA (ceRNA) microarray analysis. MiR-221(p = 0.001), miR-155(p = 0.049), and miR-130a (p = 0.001) were downregulated in CAD patients based on qRT-PCR analysis of another independent population of 932 study subjects (648 CAD subjects and 284 controls). We constructed a hsa-miR-130a-3p-mediated circRNA-mRNA ceRNA network using the miRanda database. This included 9 circRNAs (hsa_circ_0089378, hsa_circ_0083357, hsa_circ_0082824, hsa_circ_0068942, hsa_circ_0057576, hsa_circ_0054537, hsa_circ_0051172, hsa_circ_0032970, and hsa_circ_0006323) and 1 mRNA (transient receptor potential cation channel subfamily M member 3 [TRPM3]). We have shown that 9 circRNAs promote TRPM3 expression by inhibiting hsa-miR-130a-3p in CAD patients.


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