Combination of bone marrow mesenchymal stem cells sheet and platelet rich plasma for posterolateral lumbar fusion
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Zunpeng Liu1,2, Yue Zhu1, Rui Ge3, Jiajun Zhu2, Xiaoning He4, Xue Yuan5 and Xinchun Liu1
1Department of Orthopedics, First Affiliated Hospital, China Medical University, Shenyang, China
2Department of Orthopedics, Fourth Affiliated Hospital, China Medical University, Shenyang, China
3Department of Orthopedics, First Affiliated Hospital, Dalian Medical University, Dalian, China
4Department of Stomatology, Fourth Affiliated Hospital, China Medical University, Shenyang, China
5Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA, USA
Yue Zhu, email: [email protected]
Keywords: BMSC, PRP, rabbit posterolateral spinal fusion, osteogenic ability, fusion rate
Received: March 29, 2017 Accepted: June 01, 2017 Published: July 31, 2017
Bone tissue engineering provides a substitute for bone transplantation in spinal fusion. This study examined if combined bone marrow-derived mesenchymal stem cells (BMSCs) sheet with platelet-rich plasma (PRP) could promote bone regeneration in a rabbit posterolateral spinal fusion model. BMSCs was isolated and confirmed by Flow cytometric analysis and immunofluorescence staining. The morphology of BMSCs was examined by Hematoxylin and Eosin staining, scanning and transmission electron microscopy. BMSCs were cultured in induction medium or control medium. The osteogenic ability of BMSCs was investigated by various histochemical staining, immunofluorescence staining and qRT-PCR analysis. The BMSCs/PRP was constructed by encapsulating the PRP block with BMSCs sheet. Twenty-four adult rabbits were randomly divided into four groups based on the implanted biomaterials: BMSCs/PRP, BMSCs, iliac crest autograft, and control group. Manual palpation and digital radiography analysis showed that the fusion rate was 100%, 0, 83.3%, and 0 in these 4 groups, respectively. Formation of continuous bone masses in BMSCs/PRP group was confirmed by computed tomography scanning and 3D-reconstruction. These studies demonstrated that BSMCs/PRP significantly accelerated bone regeneration in the rabbit posterolateral spinal fusion model.
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