Oncotarget

Research Papers:

PLC-beta 1 regulates the expression of miR-210 during mithramycin-mediated erythroid differentiation in K562 cells

Alberto Bavelloni, Alessandro Poli, Roberta Fiume, William Blalock, Alessandro Matteucci, Giulia Ramazzotti, James A. McCubrey, Lucio Cocco and Irene Faenza _

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Oncotarget. 2014; 5:4222-4231. https://doi.org/10.18632/oncotarget.1972

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Abstract

Alberto Bavelloni1,2,*, Alessandro Poli3,*, Roberta Fiume3, William Blalock4, Alessandro Matteucci4, Giulia Ramazzotti3, James A. McCubrey5, Lucio Cocco3, Irene Faenza3

1 SC Laboratory of Musculoskeletal Cell Biology, Rizzoli Orthopedic Institute, Bologna, Italy

2 Laboratory RAMSES, Rizzoli Orthopedic Institute, Bologna, Italy

3 Cell Signaling Laboratory, Department of Biomedical Sciences, University of Bologna, Bologna, Italy

4 CNR-National Research Council of Italy, Institute of Molecular Genetics, Bologna, Italy

5 Department of Microbiology & Immunology, Brody School of Medicine, East Carolina University, Greenville, NC, USA

* These Authors contributed equally to this work

Correspondence:

Irene Faenza, email:

Lucio Cocco, email:

Keywords: phospholipase Cβ1, erythropoiesis, K562, miR-210, γ-globin

Received: April 3, 2014 Accepted: May 12, 2014 Published: May 14, 2014

Abstract

PLC-beta 1 (PLCβ1) inhibits in human K562 cells erythroid differentiation induced by mithramycin (MTH) by targeting miR-210 expression. Inhibition of miR-210 affects the erythroid differentiation pathway and it occurs to a greater extent in MTH-treated cells. Overexpression of PLCβ1 suppresses the differentiation of K562 elicited by MTH as demonstrated by the absence of γ-globin expression. Inhibition of PLCβ1 expression is capable to promote the differentiation process leading to a recovery of γ-globin gene even in the absence of MTH. Our experimental evidences suggest that PLCβ1 signaling regulates erythropoiesis through miR-210. Indeed overexpression of PLCβ1 leads to a decrease of miR-210 expression after MTH treatment. Moreover miR-210 is up-regulated when PLCβ1 expression is down-regulated. When we silenced PKCα by RNAi technique, we found a decrease in miR-210 and γ-globin expression levels, which led to a severe slowdown of cell differentiation in K562 cells and these effects were the same encountered in cells overexpressing PLCβ1. Therefore we suggest a novel role for PLCβ1 in regulating miR-210 and our data hint at the fact that, in human K562 erythroleukemia cells, the modulation of PLCβ1 expression is able to exert an impairment of normal erythropoiesis as assessed by γ-globin expression.


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