Oncotarget

Clinical Research Papers:

A three-microRNA signature for lung squamous cell carcinoma diagnosis in Chinese male patients

Lan Zhang, Xia Shan, Jun Wang, Jun Zhu, Zebo Huang, Huo Zhang, Xin Zhou, Wenfang Cheng, Yongqian Shu, Wei Zhu _ and Ping Liu

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Oncotarget. 2017; 8:86897-86907. https://doi.org/10.18632/oncotarget.19666

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Abstract

Lan Zhang1,*, Xia Shan2,*, Jun Wang3,*, Jun Zhu4, Zebo Huang1, Huo Zhang1, Xin Zhou1, Wenfang Cheng5, Yongqian Shu1, Wei Zhu1 and Ping Liu1

1Department of Oncology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China

2Department of Respiration, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing 210000, PR China

3Department of Thoracic Surgery, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China

4Department of Radiation Oncology, Jiangsu Cancer Hospital, Nanjing 210009, PR China

5Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China

*These authors have contributed equally to this work

Correspondence to:

Wei Zhu, email: zhuwei@njmu.edu.cn

Ping Liu, email: liupinga28@163.com

Keywords: serum microRNA, lung SCC, diagnostic biomarker, qRT-PCR

Received: December 21, 2016     Accepted: May 16, 2017     Published: July 28, 2017

ABSTRACT

Various studies have demonstrated the diagnostic value of microRNA (miRNA) for lung cancer, but miRNA signatures varied between different subtypes. Whether serum miRNAs could be used as biomarkers in lung squamous cell carcinoma (SCC) remains unknown. Using quantitative real-time polymerase chain reaction (qRT-PCR) based Exiqon panel, 38 differentially expressed miRNAs were identified from 3 male lung SCC pool samples and 1 normal control (NC) pool in the initial screening phase. After the training (24 SCC VS. 15 NCs), testing (44 SCC VS. 57 NCs) and external validation (34 SCC VS. 36 NCs VS. 10 pulmonary hamartoma) processes via qRT-PCR, we identified a three-miRNA panel ((miR-106a-5p, miR-20a-5p and miR-93-5p) to be a potential diagnostic marker for male lung SCC patients. The areas under the receiver operating characteristic (ROC) curve of the three-miRNA panel for the training, testing and validation phases were 0.969, 0.881 and 0.954 respectively. In addition, this signature could also differentiate lung SCC from pulmonary hamartoma (AUC=0.900). The 3 miRNAs were consistently up-regulated in lung SCC tissues (23 SCC VS. 23 NCs) and serum exosomes (17 SCC VS. 24 NCs). Moreover, expression of the 3 miRNAs was decreased in arterial serum (n = 3). In conclusion, we established a three-miRNA signature in the peripheral serum with considerable clinical value in the diagnosis of male lung SCC patients.


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