Progress and future prospect of in vitro spermatogenesis

Fahar Ibtisham, Jiang Wu, Mei Xiao, Lilong An _, Zachary Banker, Aamir Nawab, Yi Zhao and GuangHui Li

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Oncotarget. 2017; 8:66709-66727. https://doi.org/10.18632/oncotarget.19640

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Fahar Ibtisham1, Jiang Wu1, Mei Xiao1, Lilong An1, Zachary Banker2, Aamir Nawab1, Yi Zhao1 and Guanghui Li1

1 Agricultural College, Guangdong Ocean University, Zhanjiang, Guangdong, China

2 Foreign Language College, Guangdong Ocean University, Zhanjiang, Guangdong, China

Correspondence to:

Lilong An, email:

Keywords: in-vitro spermatogenesis, infertility, chemotherapy, radiotherapy

Received: March 14, 2017 Accepted: July 12, 2017 Published: July 27, 2017


Infertility has become a major health issue in the world. It affects the social life of couples and of all infertility cases; approximately 40–50% is due to “male factor” infertility. Male infertility could be due to genetic factors, environment or due to gonadotoxic treatment. Developments in reproductive biotechnology have made it possible to rescue fertility and uphold biological fatherhood. In vitro production of haploid male germ cell is a powerful tool, not only for the treatment of infertility including oligozoospermic or azoospermic patient, but also for the fertility preservation in pre-pubertal boys whose gonadal function is threatened by gonadotoxic therapies. Genomic editing of in-vitro cultured germ cells could also potentially cure flaws in spermatogenesis due to genomic mutation. Furthermore, this ex-vivo maturation technique with genomic editing may be used to prevent paternal transmission of genomic diseases. Here, we summarize the historical progress of in vitro spermatogenesis research by using organ and cell culture techniques and the future clinical application of in vitro spermatogenesis.

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