Oncotarget

Research Papers:

Use of confocal laser endomicroscopy with a fluorescently labeled fatty acid to diagnose colorectal neoplasms

Feihong Deng, Yuan Fang, Zhiyong Shen, Wei Gong, Tao Liu, Jing Wen, Wanling Zhang, Xianjun Zhu, Hui Zhong, Tong Wang, Fachao Zhi and Biao Nie _

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Oncotarget. 2017; 8:58934-58947. https://doi.org/10.18632/oncotarget.19515

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Abstract

Feihong Deng1,*, Yuan Fang1,*, Zhiyong Shen2, Wei Gong1, Tao Liu1, Jing Wen1, Wanling Zhang4, Xianjun Zhu2, Hui Zhong1, Tong Wang4, Fachao Zhi1 and Biao Nie3

1Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, 510515 China

2Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, 510515 China

3Department of Gastroenterology, First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, Guangdong Province, 510630 China

4Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong Province, 510632 China

*These authors contributed equally to this work

Correspondence to:

Biao Nie, email: niebiao2@163.com

Keywords: fatty acid metabolism, colorectal neoplasm, confocal laser endomicroscopy, fluorescent agents, de novo FA synthesis

Received: April 19, 2017     Accepted: July 11, 2017     Published: July 24, 2017

ABSTRACT

Endoscopic treatment for early colorectal cancer closely correlates with patient prognosis. However, endoscopic differentiation between carcinomas and non-neoplastic lesions remains difficult. Here, we topically stained colorectal neoplasms with a fatty acid analogue (BODIPY-FA) and quantified the fluorescent signals using confocal laser endomicroscopy (CLE) and fluorescence microscopy. We also analyzed protein expression in colorectal cancer tissues. We found that expression of fatty acid synthase was elevated, while the expression of fatty acid transporters was reduced in colorectal cancer. In colorectal cancer mouse models and patients, the BODIPY-FA signals were higher in normal epithelia than in carcinomas or colonic intraepithelial neoplasias. BODIPY-FA staining revealed both the arrangement of intestinal glands and the intracellular structures under CLE screening. In a double-blind trial, CLE images stained with BODIPY-FA exhibited greater consistency (κ = 0.68) and overall validity (74.65%) than those stained using intravenous fluorescein sodium (κ = 0.43, 55.88%) when the results were compared with histological diagnoses. These findings suggest that topical use of BODIPY-FA with CLE is a promising imaging approach for early colorectal neoplasm screening.


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