Oncotarget

Research Papers:

miR-543 promotes colorectal cancer proliferation and metastasis by targeting KLF4

Fangbing Zhai, Chunhong Cao, Liang Zhang _ and Jianhua Zhang

PDF  |  HTML  |  How to cite  |  Order a Reprint

Oncotarget. 2017; 8:59246-59256. https://doi.org/10.18632/oncotarget.19495

Metrics: PDF 1007 views  |   HTML 2147 views  |   ?  


Abstract

Fangbing Zhai1,*, Chunhong Cao1,*, Liang Zhang2 and Jianhua Zhang3

1Department of Radiology, The Second Affiliated Hospital of Dalian Medical University, Dalian 116027, Liaoning, China

2Department of Interventional Therapy, The Second Affiliated Hospital of Dalian Medical University, Dalian 116027, Liaoning, China

3Department of Nursing, The Second Affiliated Hospital of Dalian Medical University, Dalian 116027, Liaoning, China

*These authors have contributed equally to this work

Correspondence to:

Liang Zhang, email: zhangliangdlmu@163.com

Jianhua Zhang, email: zhangjianhuadlmu@163.com

Keywords: miR-543, KLF4, CRC, proliferation, metastasis

Received: May 05, 2017    Accepted: June 12, 2017    Published: July 22, 2017

ABSTRACT

Till now, miR-543 expression has been demonstrated to be involved in the development of some cancers. However, reports about its expression and mechanism in colorectal cancer (CRC) were conflicting [1, 2]. Here, we investigated clinical implications of miR-543 and mechanisms underlying miR-543-mediated CRC development. In this study, real-time quantitative PCR (qRT-PCR) validated miR-543 was highly expressed in CRC samples and cell lines. MiR-543 was closely associated with tumor size, TNM stage and metastasis. In addition, survival analysis showed that high miR-543 expression was obviously correlated with poor overall survival and disease-free survival. Mechanically, downregulation of miR-543 by miR-543 inhibitor obviously repressed cell proliferation, promoted apoptosis, affected migration and invasion. Moreover, luciferase reporter analysis identified that Krüppel-like Factor-4 (KLF4) was a direct target of miR-543, and there was an obvious inverse correlation between miR-543 and KLF4 expression in CRC tissues. Furthermore, KLF4 down-regulation favors miR-543-induced oncogenic effect on cell proliferation, apoptosis, migration, and invasion. In conclusion, this study indicated that miR-543 facilitates colorectal cancer proliferation and metastasis by targeting KLF4, and miR-543 may serve as a promising target for the treatment of CRC patients.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 19495