Protective effect of resveratrol against light-induced retinal degeneration in aged SAMP8 mice
Metrics: PDF 1222 views | HTML 3384 views | ?
Zhirong Liu1,*, Zhengzheng Wu1,*, Jie Li1, Anna Marmalidou2, Ruifan Zhang1 and Man Yu1
1Department of Ophthalmology, Hospital of University of Electronic Science and Technology of China and Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, China
2Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114, USA
*These authors have contributed equally to this work
Man Yu, email: [email protected]
Keywords: resveratrol, aging, SAMP8 mice, light damage, retinal degeneration
Received: March 13, 2017 Accepted: June 24, 2017 Published: July 22, 2017
Purpose: The purpose of this study was to determine the protective effects of Resveratrol (RESV) on acute bright light-induced retinal degeneration in aged senescence accelerated mouse strain.
Methods: Ten three-month-old male SAMP8 mice (prone to aging) were randomly assigned to two experimental dietary groups: one untreated group and one RESV treatment group (n=20 eyes for each group). After 30 days of treatment, mice were exposed to intense bright light. Ten male SAMR1 mice (resistant to aging) served as control (n=20 eyes). The protective effects of RESV administration on light-induced retinal degeneration in SAMP8 strain as well as the effect of bright light damage in the retinas of SAMP8 mice were analyzed by electroretinography (ERG), retinal histology, mRNA, protein and lipid profile.
Results: 68%-85% of a-wave amplitude and 72%-92% of b-wave amplitude were persevered by RESV in SAMP8 mice that were exposed to light damage. Also, RESV preserved their photoreceptor nuclei. mRNA expression of neuroprotective factors leukemia inhibitory factor (LIF), brain derived neurotrophic factor (BDNF), oncostatin M (OSM), cardiotrophin 1(CT-1) and cardiotrophin-like cytokine (CLC) were up-regulated 28, 8, 7, 5 and 9-fold in SAMP8 mice after RESV treatment. In addition, RESV could suppress the NF-κB pathway by down-regulating the expression of pIκB. Light damage led to increase of saturated FA, monoenoic FA, n6 PUFA and n6/n3 ratio and decrease of Docosahexaenoic acid (DHA). There was no significant difference on DHA and the ratio of n6/n3-FA between the untreated and RESV treated SAMP8 mice.
Conclusions: Collectively, our study provides evidence that RESV prevents light-induced retinal damage associated with aging.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.