Oncotarget

Research Papers:

MRGBP as a potential biomarker for the malignancy of pancreatic ductal adenocarcinoma

Feng Ding, Shuang Zhang, Shaoyang Gao, Jian Shang, Yanxia Li, Ning Cui and Qiu Zhao _

PDF  |  HTML  |  How to cite  |  Order a Reprint

Oncotarget. 2017; 8:64224-64236. https://doi.org/10.18632/oncotarget.19451

Metrics: PDF 624 views  |   HTML 1410 views  |   ?  


Abstract

Feng Ding1,2, Shuang Zhang3, Shaoyang Gao4, Jian Shang1,2, Yanxia Li5, Ning Cui5 and Qiu Zhao1,2

1Department of Gastroenterology/Hepatology, ZhongNan Hospital of Wuhan University, Wuhan 430071, China

2The Hubei Clinical Center & Key Laboratory of Intestinal & Colorectal Diseases, Wuhan 430071, China

3Laboratory of Clinical Immunology, Wuhan No.1 Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China

4Department of Pathology, Hubei Cancer Hospital, Wuhan 430079, China

5Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, China

Correspondence to:

Qiu Zhao, email: zhaoqiuwhu@sina.com

Keywords: MRGBP, pancreatic ductal adenocarcinoma, biomarker, upregulation

Received: February 24, 2017     Accepted: April 12, 2017     Published: July 22, 2017

ABSTRACT

MORF4-related gene-binding protein (MRGBP), which is also known as chromosome 20 open reading frame 20 (C20orf20), is commonly highly expressed in several types of malignant tumors and tumor progression. However, the expression pattern and underlying mechanism of MRGBP in pancreatic ductal adenocarcinoma (PDAC) remain unknown. In the study, we found that MRGBP was frequently upregulated in PDAC tissues and cell lines. In addition, the upregulation of MRGBP was positively associated with TNM stage, T classification, and poor prognosis. Knockdown of MRGBP in the PDAC cell lines ASPC-1 and Mia PaCa-2 by transiently transfected with small interfering RNA (siRNA) drastically attenuated the proliferation, migration, and invasion of those cells, whereas ectopic MRGBP overexpression in BxPC-3 cells produced exactly the opposite effect. Furthermore, we also found that overexpression of MRGBP remarkably led to cell morphological changes and induced an increased expression of mesenchymal marker Vimentin, whereas a decreased expression of epithelial marker E-cadherin. Taken together, this study indicates that MRGBP acts as a tumor oncogene in PDAC and is a promising target of carcinogenesis.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 19451