Oncotarget

Research Papers:

Vinculin and filamin-C are two potential prognostic biomarkers and therapeutic targets for prostate cancer cell migration

Jianzhong Ai, Tao Jin, Lu Yang, Qiang Wei, Yang Yang, Hong Li and Ye Zhu _

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Oncotarget. 2017; 8:82430-82436. https://doi.org/10.18632/oncotarget.19397

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Abstract

Jianzhong Ai1,2,*, Tao Jin2,1,*, Lu Yang2,1,*, Qiang Wei1,2, Yang Yang4, Hong Li1,2 and Ye Zhu3

1Institute of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan, P.R. China

2Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan, P.R. China

3Department of Cardiology, West China Hospital, Sichuan University, Chengdu, Sichuan, P.R. China

4Animal Experimental Center, West China Hospital, Sichuan University, Chengdu, Sichuan, P.R. China

*These authors have contributed equally to this work

Correspondence to:

Ye Zhu, email: [email protected]

Hong Li, email: [email protected]

Keywords: VCL, FLNC, prostate cancer, migration, quantitative proteomics

Received: April 15, 2017    Accepted: June 20, 2017    Published: July 19, 2017

ABSTRACT

Prostate cancer (PCa) is one of the most common diseases for male population, and the effective treatment for metastatic castration-resistant PCa is still lacking. To unravel the underlying mechanism of PCa cell migration, we plan to analyze the related crucial proteins and their roles. In our study, we firstly identify the differentially expressed proteins using quantitative proteomics, and confirm their mRNA expression using quantitative polymerase chain reaction (qPCR). The alterations of these proteins at DNA and mRNA levels are obtained from cBioPortal database. Furthermore, the functions of these proteins are evaluated using wound-healing assay. The quantitative proteomics identified vinculin (VCL) and filamin-C (FLNC) as two highly expressed proteins in PC3 cells, and the DNA and mRNA of these two proteins were amplified and upregulated in a part of PCa patients. Knockdown of VCL and FLNC gene expression significantly inhibit PCa cell migration. These findings suggest that VCL and FLNC identified by quantitative proteomics are highly expressed in PCa cells with high migration potential, and they could be effective targets for repressing PCa cell migration, paving a new avenue for the prognosis and treatment of advanced PCa.


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