Altered microRNAs expression in T cells of patients with SLE involved in the lack of vitamin D
Metrics: PDF 1100 views | HTML 1500 views | ?
Dao-Jun Chen1,2, Lan-Ju Li1,2, Xiao-Ke Yang1,2, Tao Yu1,2, Rui-Xue Leng1,2, Hai-Feng Pan1,2 and Dong-Qing Ye1,2
1Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, Anhui, China
2Anhui Province Key Laboratory of Major Autoimmune Diseases, Hefei, Anhui, China
Keywords: microRNA, vitamin D, SLE, T cells
Received: August 15, 2016 Accepted: May 31, 2017 Published: July 07, 2017
Vitamin D has been recognized as a potent immunomodulator and its deficiency is common in different population groups including patients with SLE. As miRNAs regulation plays a significant role in SLE, the present study aimed to evaluate the association between vitamin D status and miRNAs levels in patients with SLE. The serum concentrations of vitamin D (25-hydroxyvitamin D) and the levels of six miRNAs in T cells from patients with SLE were measured in 42 SLE cases and 48 healthy controls. Vitamin D treatment was also performed in isolated and cultured T cells from SLE patients in different times and doses. Vitamin D insufficiency (25-hydroxyvitamin D concentration<20 ng/ml) was more common in cases than in controls. Although age and BMI were similar, cases had significantly lower concentrations of miRNA-377, miRNA-342, miRNA-10a, miRNA-374b, miRNA-125a, and miRNA-410 than controls. Furthermore, a significant positive correlation was also observed between 25-hydroxyvitamin D concentrations and measured miRNAs levels. A significant difference in observed miRNAs levels was also observed in patients with 25-hydroxyvitamin D insufficiency compared with patients with 25-hydroxyvitamin D concentration≥20 ng/ml. And 1α,25(OH)2D3 differentially regulated miRNAs expression in dose- and time- manner in vitro. Lower expressions of miRNA-377, miRNA-342, miRNA-10a, miRNA-374b, miRNA-125a, and miRNA-410 were found in SLE patients. And severe vitamin D deficiency is associated with decreased observed miRNAs levels in SLE patients. A 25-hydroxyvitamin D concentration value<20 ng/ml is suggested as the “cut-off” for such immunological alterations in patients with SLE.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.