Oncotarget

Research Papers:

Impact of chronic methamphetamine treatment on the atherosclerosis formation in ApoE−/− mice fed a high cholesterol diet

Pengfei Zhu, Lun Li, Bo Gao, Mingjing Zhang, Yuting Wang, Ye Gu and Liqun Hu _

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Oncotarget. 2017; 8:55064-55072. https://doi.org/10.18632/oncotarget.19020

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Abstract

Pengfei Zhu1,*, Lun Li1,*, Bo Gao1, Mingjing Zhang1, Yuting Wang1, Ye Gu1 and Liqun Hu1

1Department of Cardiology, Heart Center at Puai Hospital, Puai Hospital, Huazhong University of Science and Technology, Wuhan 430030, China

*These authors contributed equally to this work

Correspondence to:

Liqun Hu, email: [email protected]

Keywords: atherosclerosis, methamphetamine, immune balance, inflammation

Received: March 16, 2017     Accepted: June 04, 2017     Published: July 05, 2017

ABSTRACT

Background: We previously reported that methamphetamine could promote atherosclerosis (AS) in ApoE−/− mice fed normal chow. We herein observed the impact of methamphetamine on AS in ApoE−/− mice fed a high cholesterol diet and explored the potential mechanisms.

Results and Materials and Methods: Male ApoE−/− mice fed a high cholesterol diet were treated with saline (NS, n = 5) or methamphetamine [8 mg/kg/day (M8, n = 6) through intraperitoneal injection] for 24 weeks. Afterwards, the percentage area of atheromatous plaque in aortic root (44.31 ± 3.21% vs. 32.91 ± 3.58%, P < 0.01) and atherosclerotic lesion area on Oil red O stained en face aorta (32.74 ± 6.97% vs. 18.72 ± 3.65%, P < 0.01) were significantly higher in M8 group than in NS group. The percentages of Th1 cells and Th17 cells in spleen were significantly higher while the percentages of Th2 cells and CD4+CD25+Foxp3+ Tregs were significantly lower in M8 group than in NS group. mRNA expressions of TNF-α, IFN-γ, and IL-17 were significantly up-regulated , IL-4, IL-10, Foxp3, and TGF-β were significantly down-regulated in carotid artery and in spleen in M8 group compared to NS group.

Conclusions: Chronic methamphetamine treatment can enhance atherosclerotic plaque formation possibly through promoting proinflammatory cytokine secretions in ApoE−/− mice fed a high cholesterol diet.


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