Oncotarget

Research Papers:

Potential genotoxic and cytotoxicity of emamectin benzoate in human normal liver cells

Zhijie Zhang, Xinyu Zhao and Xiaosong Qin _

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Oncotarget. 2017; 8:82185-82195. https://doi.org/10.18632/oncotarget.18988

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Abstract

Zhijie Zhang1,*, Xinyu Zhao2,* and Xiaosong Qin1

1Clinical Laboratory, Shengjing Hospital, China Medical University, Shenyang 110011, China

2Complex Laboratory, College of Laboratory Medicine, Dalian Medical University, Dalian 116044, China

*These authors have contributed equally to this work

Correspondence to:

Xiaosong Qin, email: [email protected]

Keywords: emamectin benzoate, human liver cells, potential genotoxic, cytotoxic effects, mitochondrial-mediated apoptotic

Received: April 19, 2017     Accepted: May 23, 2017     Published: July 04, 2017

ABSTRACT

Pesticide residue inducing cancer-related health problems draw people more attention recently. Emamectin benzoate (EMB) has been widely used in agriculture around the world based on its specificity targets. Although potential risk and the molecular mechanism of EMB toxicity to human liver has not been well-characterized. Unlike well-reported toxicity upon central nervous system, potential genotoxic and cytotoxicity of EMB in human liver cell was ignored and very limited. In this study, we identify genotoxicity and cytotoxicity of EMB to human normal liver cells (QSG7701 cell line) in vitro. We demonstrate that EMB inhibited the viability of QSG7701 cells and induced the DNA damage. Established assays of cytotoxicity were performed to characterize the mechanism of EMB toxicity on QSG7701 cells. Typical chromatin condensation and DNA fragmentation indicated the apoptosis of QSG7701 cells induced by EMB. And the intracellular biochemical results demonstrated that EMB-enhanced apoptosis of QSG7701 cells concurrent with generated ROS, a loss of mitochondrial membrane potential, the cytochrome-c release, up regulate the Bax/Bcl-2 and the activation of caspase-9/-3. Our results of EMB induces the death of QSG7701 cells maybe via mitochondrial-mediated intrinsic apoptotic pathways would contribute to promote the awareness of EMB as an extensive used pesticide to human being effects and reveal the underlying mechanisms of potential genotoxic.


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