Presence of embryonic DNA in culture medium
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Linlin Yang1,2,*, Qiaoying Lv3,*, Wei Chen1, Jian Sun1, Yu Wu1, Yiying Wang4, Xiong Chen2, Xiaojun Chen3 and Zhenbo Zhang1,2
1The Reproductive Medicine Center of Department of Obstetrics and Gynecology, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai 200080, China
2Department of Obstetrics and Gynecology, Shanghai First People’s Hospital, Baoshan Branch, Shanghai 201900, China
3Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China
4Department of Obstetrics and Gynecology, Henan Province People’s Hospital, Zhengzhou 450000, China
*These authors have contributed equally to this work
Zhenbo Zhang, email: [email protected]
Keywords: embryonic culture medium, intracytoplasmic sperm injection, polymerase chain reaction, preimplantation genetic diagnosis, in vitro fertilization
Received: April 14, 2017 Accepted: June 01, 2017 Published: June 29, 2017
Preimplantation genetic diagnosis (PGD) has successfully assisted couples with genetic diseases to conceive healthy babies during the past decades. However, biopsy of the blastomere has potential lesion to the embryos which commonly results in abortion. Thus, a noninvasive PGD is needed. In the past, the presence of genetic materials in maternal plasma or serum has triggered a great innovation of noninvasive prenatal diagnosis. Nevertheless, it is not clear whether embryonic DNA is also present in embryonic culture medium. Here, a rapid-boiling method has been used to harvest DNA from the medium or the discarded embryos, following Polymerase Chain Reaction (PCR) was applied to detect the dissociative DNA by amplifying SRY gene (Y-chromosome). For the first time, the Y sequences were detected in the medium which were used to culture embryo for above 3 days. None of the positive signal was examined in Day 1 and Day 2 embryonic culture medium. Our findings suggest that the Y chromosome fragments from the embryo may release into its culture medium. If validated in a larger cohort, detection of SRY gene may prove to be a useful method to screen Y-linked genetic disease. More importantly, detecting the free DNA in the embryonic culture medium may represent a novel strategy for noninvasive PGD.
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