Gut microbial profile analysis by MiSeq sequencing of pancreatic carcinoma patients in China
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Zhigang Ren1,3,4,*, Jianwen Jiang1,3,*, Haiyang Xie1,3, Ang Li2,3,4, Haifeng Lu2,3, Shaoyan Xu1,3, Lin Zhou1,3, Hua Zhang2,3, Guangying Cui2,3,4, Xinhua Chen1,3, Yuanxing Liu1,3, Liming Wu1,3, Nan Qin2,3, Ranran Sun4, Wei Wang1,3, Lanjuan Li2,3, Weilin Wang1,3 and Shusen Zheng1,3
1Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, Hangzhou 310003, China
2State Key Laboratory for Diagnosis and Treatment of Infectious Disease, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China
3Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310003, China
4Department of Infectious Diseases, Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
*These authors contributed equally to this work
Shusen Zheng, email: [email protected]
Weilin Wang, email: [email protected]
Keywords: pancreatic carcinoma, gut microbiota, MiSeq sequencing, alpha diversity, biomarkers
Received: April 23, 2017 Accepted: June 10, 2017 Published: June 29, 2017
Pancreatic carcinoma (PC) is a lethal cancer. Gut microbiota is associated with some risk factors of PC, e.g. obesity and types II diabetes. However, the specific gut microbial profile in clinical PC in China has never been reported. This prospective study collected 85 PC and 57 matched healthy controls (HC) to analyze microbial characteristics by MiSeq sequencing. The results showed that gut microbial diversity was decreased in PC with an unique microbial profile, which partly attributed to its decrease of alpha diversity. Microbial alterations in PC featured by the increase of certain pathogens and lipopolysaccharides-producing bacteria, and the decrease of probiotics and butyrate-producing bacteria. Microbial community in obstruction cases was separated from the un-obstructed cases. Streptococcus was associated with the bile. Furthermore, 23 microbial functions e.g. Leucine and LPS biosynthesis were enriched, while 13 functions were reduced in PC. Importantly, based on 40 genera associated with PC, microbial markers achieves a high classification power with AUC of 0.842. In conclusion, gut microbial profile was unique in PC, providing a microbial marker for non-invasive PC diagnosis.
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