Insulin resistance in vitamin D-deficient mice is alleviated by n-acetylcysteine
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Zhao-Hui Cui1,*, Qi Yuan1,*, Li Mao1, Feng-Li Chen2, Feng Ji3 and Sha Tao1
1Department of Endocrinology, Huai’an First People’s Hospital, Nanjing Medical University, Huai’an, China
2Clinical Laboratory, Huai’an First People’s Hospital, Nanjing Medical University, Huai’an, China
3Department of Orthopedics, Huai’an First People’s Hospital, Nanjing Medical University, Huai’an, China
*These authors are considered as co-first authors
Li Mao, email: [email protected]
Feng Ji, email: [email protected]
Keywords: vitamin D, insulin resistance, oxidative stress, N-acetylcysteine (NAC), inflammation
Received: May 07, 2017 Accepted: May 23, 2017 Published: June 28, 2017
Vitamin D deficiency will lead to insulin resistance. In the current study, vitamin D3 1α-Hydroxylase [“1α(OH)ase”] knockout mice were generated to mimic vitamin D deficiency in vivo. As compared to the wild-type mice, the liver tissues of the knockout mice showed impaired insulin signaling, decreased glucose transporter 4 expression and increased reactive oxygen species production. Meanwhile, p53-p21 activation, apoptosis intensity and pro-inflammatory cytokines (IL-6, IL-1 and MIP-1α) level were significantly increased in the knockout mice livers. Significantly, such effects in the knockout mice were largely attenuated by supplement with anti-oxidant n-acetylcysteine (NAC). Remarkably, insulin resistance and metabolic abnormalities in the knockout mice were largely alleviated after treatment of NAC. Therefore, inhibition of oxidative stress by NAC alleviated insulin resistance in vitamin D-deficient mice. Oxidative stress could be the primary cause of insulin resistance by vitamin D deficiency.
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