Clinical Research Papers:
Association study of inflammatory cytokine and chemokine expression in hand foot and mouth disease
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Wenzhong Shang1,*, Suying Qian2,*, Lijuan Fang3, Yong Han4 and Cuiping Zheng5
1People’s Hospital of Fuyang District, Hangzhou, Zhejiang, P. R. China
2The Second Hospital of Ningbo, Ningbo, Zhejiang, P. R. China
3The Fourth Hospital of Xiaoshan District, Hangzhou, Zhejiang, P. R. China
4Key Laboratory of Tumor Molecular Diagnosis and Individualized Medicine of Zhejiang Province, Zhejiang Provincial People’s Hospital, Hangzhou, Zhejiang, P. R. China
5Dingli Hospital of Wenzhou Medical School, Wezhou Central Hospital, Zhejiang, P. R. China
*These authors contributed equally to this work
Cuiping Zheng, email: [email protected]
Yong Han, email: [email protected]
Keywords: hand, foot and mouth disease, enterovirus 71, inflammatory cytokines, chemokines
Received: June 17, 2016 Accepted: May 23, 2017 Published: June 02, 2017
Objective: To determine the relationship of cytokine/chemokine expression with the clinical presentation of hand, foot and mouth disease (HFMD).
Results: All cytokine/chemokine levels were higher in severe HFMD patients than in mild HFMD patients or controls (P < 0.01). RANTES, MCP-1, IL-4, IL-12 and IL-18 levels were higher in mild HFMD patients than in the controls (P < 0.05). In severe HFMD, all levels (except IL-8 and IL-4) were higher in patients with encephalitis plus pulmonary edema than in those with encephalitis alone (P < 0.05). All levels (except IL-8) were higher in EV71-positive patients than in EV71-negative patients (P < 0.05). In mild HFMD, all levels (except IL-8 and IL-4) were higher in EV71-positive patients than in EV71-negative patients (P < 0.05). In severe HFMD, only RANTES, IP-10 and IFN-γ levels were higher in EV71-positive patients than in EV71-negative patients (P < 0.05). In the EV71-negative group, all levels were higher in severe HFMD than in mild HFMD (P < 0.01). In the EV71-positive group, all levels (except IL-8) were higher in severe HFMD than in mild HFMD (P < 0.01).
Materials and Methods: This study involved 28 mild HFMD patients, 44 severe HFMD patients and 26 healthy children. Venous blood was tested for cytokines (IL-4, IL-12, IL-18, TNF-α, IFN-γ) and chemokines (IL-8, RANTES, MCP-1, IP-10). Stool samples from the patients were tested for EV71 nucleic acid using reverse transcription polymerase chain reaction.
Conclusions: Cytokines/chemokines participate in HFMD pathogenesis, and could have potential value in monitoring disease progression and predicting prognosis.
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