Research Papers:

Expression of LAG-3 defines exhaustion of intratumoral PD-1+ T cells and correlates with poor outcome in follicular lymphoma

Zhi-Zhang Yang _, Hyo Jin Kim, Jose C. Villasboas, Ya-Ping Chen, Tammy Price-Troska, Shahrzad Jalali, Mara Wilson, Anne J. Novak and Stephen M. Ansell

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Oncotarget. 2017; 8:61425-61439. https://doi.org/10.18632/oncotarget.18251

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Zhi-Zhang Yang1, Hyo Jin Kim1, Jose C. Villasboas1, Ya-Ping Chen2, Tammy Price-Troska1, Shahrzad Jalali1, Mara Wilson1, Anne J. Novak1 and Stephen M. Ansell1

1Division of Hematology and Internal Medicine, Mayo Clinic, Rochester, MN, USA

2Division of Oncology/Hematology and Internal Medicine, College of Medicine, National Cheng Kung University and Hospital, Tainan, Taiwan

Correspondence to:

Zhi-Zhang Yang, email: [email protected]

Stephen M. Ansell, email: [email protected]

Keywords: follicular lymphoma, LAG-3, T-cell exhaustion, PD-1, immune checkpoint

Received: March 21, 2017     Accepted: April 26, 2017     Published: May 29, 2017


Exhausted T-cells in follicular lymphoma (FL) typically express PD-1, but expression of PD-1 is not limited to exhausted cells. Although expected to be functionally suppressed, we found that the population of intratumoral PD-1+ T cells were predominantly responsible for production of cytokines and granules. This surprising finding prompted us to explore the involvement of LAG-3 to specifically identify functionally exhausted T cells. We found that LAG-3 was expressed on a subset of intratumoral T cells from FL and LAG-3+ T cells almost exclusively came from PD-1+ population. CyTOF analysis revealed that intratumoral LAG-3+ T cells were phenotypically heterogeneous as LAG-3 was expressed on a variety of T cell subsets. In contrast to PD-1+LAG-3- cells, intratumoral PD-1+LAG-3+ T cells exhibited reduced capacity to produce cytokines and granules. LAG-3 expression could be substantially upregulated on CD4+ or CD8+ T cells by IL-12, a cytokine that has been shown to induce T-cell exhaustion and be increased in the serum of lymphoma patients. Furthermore, we found that blockade of both PD-1 and LAG-3 signaling enhanced the function of intratumoral CD8+ T cells resulting in increased IFN-γ and IL-2 production. Clinically, LAG-3 expression on intratumoral T cells correlated with a poor outcome in FL patients. Taken together, we find that LAG-3 expression is necessary to identify the population of intratumoral PD-1+ T cells that are functionally exhausted and, in contrast, find that PD-1+LAG-3- T cells are simply activated cells that are immunologically functional. These findings may have important implications for immune checkpoint therapy in FL.

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