Research Papers: Immunology:
Trans-sialidase-based vaccine candidate protects against Trypanosoma cruzi infection, not only inducing an effector immune response but also affecting cells with regulatory/suppressor phenotype
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Estefanía Prochetto1, Carolina Roldán1, Iván A. Bontempi1,2, Daiana Bertona1, Luz Peverengo1, Miguel H. Vicco2, Luz M. Rodeles2, Ana R. Pérez3, Iván S. Marcipar1,2 and Gabriel Cabrera1,2
1 Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina
2 Facultad de Ciencias Médicas, Universidad Nacional del Litoral, Santa Fe, Argentina
3 IDICER-CONICET and Instituto de Inmunología, Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Santa Fe, Argentina
Gabriel Cabrera, email:
Keywords: Trypanosoma cruzi, trans-sialidase, vaccine, foxp3 regulatory T cells, myeloid-derived suppressor cells, Immunology and Microbiology Section, Immune response, Immunity
Received: March 21, 2017 Accepted: May 08, 2017 Published: May 25, 2017
Prophylactic and/or therapeutic vaccines have an important potential to control Trypanosoma cruzi (T. cruzi)infection. The involvement of regulatory/suppressor immune cells after an immunization treatment and T. cruzi infection has never been addressed. Here we show that a new trans-sialidase-based immunogen (TSf) was able to confer protection, correlating not only with beneficial changes in effector immune parameters, but also influencing populations of cells related to immune control.
Regarding the effector response, mice immunized with TSf showed a TS-specific antibody response, significant delayed-type hypersensitivity (DTH) reactivity and increased production of IFN-γ by CD8+ splenocytes. After a challenge with T. cruzi, TSf-immunized mice showed 90% survival and low parasitemia as compared with 40% survival and high parasitemia in PBS-immunized mice.
In relation to the regulatory/suppressor arm of the immune system, after T. cruzi infection TSf-immunized mice showed an increase in spleen CD4+ Foxp3+ regulatory T cells (Treg) as compared to PBS-inoculated and infected mice. Moreover, although T. cruzi infection elicited a notable increase in myeloid derived suppressor cells (MDSC) in the spleen of PBS-inoculated mice, TSf-immunized mice showed a significantly lower increase of MDSC.
Results presented herein highlight the need of studying the immune response as a whole when a vaccine candidate is rationally tested.
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