Oncotarget

Research Papers:

By up-regulating μ- and δ-opioid receptors, neuron-restrictive silencer factor knockdown promotes neurological recovery after ischemia

Hui-Min Liang _, Li-Jiao Geng, Xiao-Yan Shi, Chao-Gang Zhang, Shu-Yan Wang and Guang-Ming Zhang

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Oncotarget. 2017; 8:101012-101025. https://doi.org/10.18632/oncotarget.18195

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Abstract

Hui-Min Liang1,*, Li-Jiao Geng1,*, Xiao-Yan Shi2, Chao-Gang Zhang1, Shu-Yan Wang3 and Guang-Ming Zhang3

1Department of Neurology, Huaihe Hospital of Henan University, Kaifeng 475000, China

2Institute of Traditional Chinese Medicine, Henan University, Kaifeng 475000, China

3Department of Anesthesiology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China

*These authors contributed equally to this work

Correspondence to:

Hui-Min Liang, email: minhh_ll@163.com

Keywords: neuron-restrictive silencer factor, cerebral ischemia, μ- and δ-opioid receptors, proliferation, apoptosis

Received: February 06, 2017     Accepted: May 06, 2017     Published: May 23, 2017

ABSTRACT

We investigated the effects of neuron-restrictive silencer factor (NRSF) on proliferation of endogenous nerve stem cells (NSCs) and on μ- and δ-opioid receptor (MOR/DOR) expression in rats after cerebral ischemia. Among 100 rats subjected to cerebral ischemia, 20 rats were transfected with NRSF shRNA, and the remaining 80 were randomly assigned to normal, sham, model, and negative control (NC) groups. On days 7, 14, and 28 after ischemia and reperfusion, neurological function scores were assigned and a step-down passive avoidance test was conducted. Nerve function scores, step-down reaction periods, error times and apoptosis rates, as well as levels of B-cell CLL/lymphoma 2 (Bcl-2), BCL2-associated X protein (Bax), and NRSF expression were lower in the NRSF shRNA group than in the model and NC groups. By contrast, step-down latency, numbers of bromodeoxyuridine-positive cells, MOR/DOR expression, and phosphorylation of extracellular signal regulated protein kinase (ERK) and cAMP response element binding protein (CREB) were higher in the NRSF shRNA group than in the model and NC groups. These results suggest that by up-regulating MOR/DOR expression, NRSF knockdown accelerates recovery of neurological function after cerebral ischemia, at least in part by promoting NSC proliferation and inhibiting apoptosis.


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