Accumulation of prohibitin is a common cellular response to different stressing stimuli and protects melanoma cells from ER stress and chemotherapy-induced cell death
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Tharcisio Citrangulo Tortelli Junior1, Lyris Martins Franco de Godoy2,4,6, Gustavo Antonio de Souza2,5,6, Diego Bonatto3, Andreia Hanada Otake1, Renata de Freitas Saito1, Jose Cesar Rosa2,6, Lewis Joel Greene2,6 and Roger Chammas1
1Centro de Investigação Translacional em Oncologia (LIM24), Departamento de Radiologia e Oncologia, Faculdade de Medicina da Universidade de São Paulo and Instituto do Câncer do Estado de São Paulo, São Paulo, SP, Brazil
2Departamento de Biologia Celular, Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil
3Centro de Biotecnologia, Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
4Instituto Carlos Chagas, Fiocruz Paraná, PR, Brazil
5Instituto do Cérebro, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil
6Centro de Terapia Celular, Fundação Hemocentro de Ribeirão Preto, Ribeirão Preto, SP, Brazil
Roger Chammas, email: email@example.com
Lewis Joel Greene, email: firstname.lastname@example.org
Keywords: melanoma, prohibitin, mitochondria, cisplatin, tunicamycin
Received: December 23, 2016 Accepted: April 19, 2017 Published: May 11, 2017
Melanoma is responsible for most deaths among skin cancers and conventional and palliative care chemotherapy are limited due to the development of chemoresistance. We used proteomic analysis to identify cellular responses that lead to chemoresistance of human melanoma cell lines to cisplatin. A systems approach to the proteomic data indicated the participation of specific cellular processes such as oxidative phosphorylation, mitochondrial organization and homeostasis, as well as the unfolded protein response (UPR) to be required for the survival of cells treated with cisplatin. Prohibitin (PHB) was among the proteins consistently accumulated, interacting with the functional clusters associated with resistance to cisplatin. We showed PHB accumulated at different levels in melanoma cell lines under stressing stimuli, such as (i) treatment with temozolomide (TMZ), dacarbazine (DTIC) and cisplatin; (ii) serum deprivation; (iii) tunicamycin, an UPR inducer. Prohibitin accumulated in the mitochondria of melanoma cells after cisplatin and tunicamycin treatment and its de novo accumulation led to chemoresistance melanoma cell lines. In contrast, PHB knock-down sensitized melanoma cells to cisplatin and tunicamycin treatment. We conclude that PHB participates in the survival of cells exposed to different stress stimuli, and can therefore serve as a target for the sensitization of melanoma cells to chemotherapy.
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