MGr1-Antigen/37 kDa laminin receptor precursor promotes cellular prion protein induced multi-drug-resistance of gastric cancer
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Guanhong Luo1,*, Weijie Wang1,*, Qiong Wu1,*, Yuanyuan Lu1,*, Tao Su2, Nan Gu3, Kai Li1, Jingbo Wang1, Rui Du4, Xiaodi Zhao1, Xiaohua Li1, Rui Fan1, Hongbo Zhang1, Yongzhan Nie1, Xinmin Zhou1, Yongquan Shi1, Jie Liang1, Xin Wang1 and Daiming Fan1
1State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi'an, China
2Department of Cardiology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China
3Department of Anesthesiology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China
4Department of Radiotherapy Oncology, Navy General Hospital, Beijing, China
*These authors contributed equally to this work
Jie Liang, email: [email protected]
Xin Wang, email: [email protected]
Daiming Fan, email: [email protected]
Keywords: PrPC, MGr1-Ag/37LRP, gastric cancer, multi-drug-resistance (MDR), apoptosis
Received: August 02, 2016 Accepted: April 24, 2017 Published: May 11, 2017
Cellular prion protein (PrPC), the infective agent of transmissible spongiform encephalopathies, is thought to be related to several cellular physiological and physiopathological processes. We have previously reported that PrPC participates in multi-drug-resistance of gastric cancer. As the salient ligand molecule of PrP for participating in internalization and propagation of the scrapie form of prion protein (PrPSc), 37 kDa laminin receptor precursor protein (37LRP) shared the same gene coding sequence of MGr1-Ag, another protein previously found to be involved in multi-drug-resistance of gastric cancer in our lab. In the present study, we explored whether MGr1-Ag/37LRP contributed to PrPC mediated multi-drug-resistance in gastric cancer. Immunohistochemical staining showed similar expression patterns of MGr1-Ag/37LRP and PrPC in gastric cancer tissue serial sections. Western blot and immunohistochemistry also demonstrated correlative expression of MGr1-Ag/37LRP and PrPC in gastric cancer cell lines. Interaction between MGr1-Ag/37LRP and PrPC in gastric cancer cell lines and gastric cancer tissues were verified by immunofluorescence and co-immunoprecipitation. Furthermore, knockdown of MGr1-Ag/37LRP significantly attenuated PrPC induced multi-drug-resistance by sensitizing drug-induced apoptosis through inhibition of AKT activation. In conclusion, MGr1-Ag/37LRP may interact with PrPC and promote the PrPC induced multi-drug-resistance in gastric cancer through PI3K/AKT pathway. The current study elucidates the mechanism of how PrPC triggers intracellular signaling cascade resulting in multi-drug-resistance phenotype and provides a novel candidate molecular target against gastric cancer.
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