Oncotarget

Research Papers:

Overexpression of a novel candidate oncogene KIF14 correlates with tumor progression and poor prognosis in prostate cancer

Yixiang Zhang, Yeqing Yuan, Pei Liang, Zhaoxia Zhang, Xiaojing Guo, Ligang Xia, Yingying Zhao, Xing-Sheng Shu, Shengkun Sun, Ying Ying and Yingduan Cheng _

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Oncotarget. 2017; 8:45459-45469. https://doi.org/10.18632/oncotarget.17564

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Abstract

Yixiang Zhang1,*, Yeqing Yuan1,*, Pei Liang2,*, Zhaoxia Zhang3, Xiaojing Guo4, Ligang Xia5, Yingying Zhao6, Xing-Sheng Shu7, Shengkun Sun8, Ying Ying6 and Yingduan Cheng1

1Department of Urology, The Second Affiliated Hospital of Jinan University, Shenzhen People’s Hospital, Shenzhen, Guangdong, People’s Republic of China

2Department of Urology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA

3Department of Pediatrics, The Second Affiliated Hospital of Jinan University, Shenzhen People’s Hospital, Shenzhen, Guangdong, People’s Republic of China

4Department of Pathology, The Second Affiliated Hospital of Jinan University, Shenzhen People’s Hospital, Shenzhen, Guangdong, People’s Republic of China

5Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Jinan University, Shenzhen People’s Hospital, Shenzhen, Guangdong, People’s Republic of China

6Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen, Guangdong, People’s Republic of China

7Institute of Molecular Medicine, Health Science Center, Shenzhen University, Shenzhen, Guangdong, People’s Republic of China

8Department of Urology, Chinese PLA General Hospital, Beijing, People’s Republic of China

*These authors contributed equally to this work

Correspondence to:

Yingduan Cheng, email: [email protected]

Ying Ying, email: [email protected]

Keywords: KIF14, prostate cancer, apoptosis, proliferation, G2 arrest

Received: March 09, 2017     Accepted: April 18, 2017     Published: May 02, 2017

ABSTRACT

Prostate cancer (PCa) is the second leading cause of death from cancer in men. The mechanism underlying tumorigenesis and development of PCa is largely unknown. Here, we identified Kinesin family member 14 (KIF14) as a novel candidate oncogene in PCa. We found that KIF14 was overexpressed in multiple PCa cell lines and primary PCa tissues. Knockdown of KIF14 in DU145 and PC3 prostate cancer cells suppressed cell proliferation, induced cell cycle arrest and apoptosis. Transcriptome analysis by RNA-sequencing demonstrated that KIF4 suppression led to transcriptional changes of genes involved in p53 and TGF-beta signaling pathway. In addition, upregulated expression of GADD45A, GADD45B, p21, PIDD and Shisa5, which contribute to growth arrest and apoptosis induction, and downregulated CCNB1 that promotes cell cycle progression were confirmed by quantitative real-time PCR after KIF4 knockdown. We further found that KIF14 protein level was positively correlated with T stage and Gleason Score. Patients with higher KIF14 expression had shorter overall survival time than those with lower KIF14 expression. Thus, our data indicate that KIF14 could act as a potential oncogene that contributes to tumor progression and poor prognosis in PCa, which may represent a novel and useful prognostic biomarker for PCa.


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