MiR-187 overexpression inhibits cervical cancer progression by targeting HPV16 E6
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Mao Lin1, Xiang-Yang Xue2, Shu-Zhen Liang1, Yin-Xiong Li3, You-Yong Lv4, Li-Hua He1, Ke-Cheng Xu1, Li-Fang Zhang2, Ji-Bing Chen1 and Li-Zhi Niu1
1Fuda Cancer Hospital, Jinan University School of Medicine, Department of Central Laboratory, Guangzhou, China
2Wenzhou Medical University, Department of Immunology and Microbiology, Wenzhou, China
3Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
4Peking University Cancer Hospital, Department of Central Laboratory, Beijing, China
Ji-Bing Chen, email: [email protected]
Li-Fang Zhang, email: [email protected]
Keywords: microRNA-187, HPV16 E6, cervical cancer, occurrence, progression
Received: November 25, 2016 Accepted: April 11, 2017 Published: April 29, 2017
Aberrantly expressed microRNAs contribute to the initiation and progression of human cancer. MiRNA-187 has been reported in nasopharyngeal, renal, pancreatic, prostate, and esophageal cancer, and acts as a tumor suppressor or oncogene. However, the underlying function of miRNA-187 in cervical cancer remains largely unexplored. In the present study, we demonstrated significantly miRNA-187 down-regulation in cervical cancer tissues and cell lines compared to their normal counterparts. Kaplan-Meier analysis revealed that decreased miRNA-187 was closely associated with shorter overall survival and relapse-free survival. Gain- and loss-of-function studies showed that miRNA-187 suppressed cervical cancer cell proliferation, migration, and invasion, and promoted cervical cancer cell apoptosis. Furthermore, luciferase reporter assay determined that human papillomavirus 16 E6 was a direct functional target of miRNA-187. Taken together, our findings indicate the essential role of miRNA-187 in suppressing cervical cancer progression and indicate a novel link between miRNA-187 and human papillomavirus 16 E6 in cervical cancer.
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