Research Papers:

Multi-modality imaging-monitored creation of rat orthotopic pancreatic head cancer with obstructive jaundice

Zhibin Bai, Yaoping Shi, Jianfeng Wang, Longhua Qiu, Gaojun Teng, Feng Zhang and Xiaoming Yang _

PDF  |  HTML  |  How to cite

Oncotarget. 2017; 8:54277-54284. https://doi.org/10.18632/oncotarget.17347

Metrics: PDF 1963 views  |   HTML 2497 views  |   ?  


Zhibin Bai1,2, Yaoping Shi1, Jianfeng Wang1, Longhua Qiu1, Gaojun Teng2, Feng Zhang1,* and Xiaoming Yang1,3,*

1Image-Guided Biomolecular Intervention Research, Section of Interventional Radiology, Department of Radiology, University of Washington School of Medicine, Seattle, WA, USA

2Department of Radiology, Zhongda Hospital, Southeastern University, Nanjing, China

3Department of Radiology, Sir Run Run Show Hospital, Zhejiang University School of Medicine, Hangzhou, China

*These authors contributed equally to this work

Correspondence to:

Xiaoming Yang, email: [email protected]

Keywords: pancreatic cancer, multi-modality imaging, model, rat, orthotopic

Received: January 18, 2017     Accepted: April 07, 2017     Published: April 21, 2017


Purpose: To investigate the feasibility of using multi-modality imaging to monitor the creation of rat models with orthotopic pancreatic head cancer with obstructive jaundice.

Results: 27 of 52 rats (51.92%) developed pancreatic head cancer. The tumor formation rate was significantly higher in the animal group receiving bioluminescent tumor, compared to the group receiving non-bioluminescent donor tumors [78.1% (25/32 rats) vs 10.0% (2/20 rats), P = 0.0001]. Both ultrasound imaging and MRI clearly characterized the orthotopic tumors. Laboratory biochemistry test for those rats with obstructive jaundice showed elevated levels of bilirubin, aspartate transaminase (AST), alkaline phosphatase (ALT) and gamma-glutamyl transpeptidase (λ-GGT), compared with those rats without jaundice (P < 0.05). Correlative pathology confirmed that all tumors were ductal adenocarcinomas, and located in pancreatic head regions.

Materials and Methods: Rat pancreatic adenocarcinoma cells (DSL-6A/C1) were first transfected with lentivirus/mCherry-luciferase genes, and then subcutaneously implanted into flanks of donor immunocompetent Lewis rats, to create pancreatic tumor tissues. The tumor tissues from donor rats with either bioluminescence signal or without the signal were then transplanted into the pancreatic heads of 52 recipient Lewis rats. Bioluminescence optical and ultrasound imaging, as well as magnetic resonance imaging (MRI), were performed to follow up the tumor formation and growth in these tumor-transplanted rats. Physical examination and biochemistry test were used to discern the rats with obstructive jaundice. The rats were euthanized for subsequent histologic correlation and confirmation.

Conclusions: We successfully created a new rat model with orthotopic pancreatic head cancer, which can be accurately monitored and visualized by different imaging modalities.

Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 17347