Oncotarget

Research Papers:

TrkC promotes colorectal cancer growth and metastasis

Min Soo Kim, Kwang Wook Suh, Suntaek Hong and Wook Jin _

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Oncotarget. 2017; 8:41319-41333. https://doi.org/10.18632/oncotarget.17289

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Abstract

Min Soo Kim1, Kwang Wook Suh2, Suntaek Hong3 and Wook Jin1,4

1Laboratory of Molecular Disease and Cell Regulation, Department of Biochemistry, School of Medicine, Gachon University, Incheon 406-840, Korea

2Department of Surgery, Ajou University School of Medicine, Yeongto-gu, Suwon 443-380, Korea

3Laboratory of Cancer Cell Biology, Department of Biochemistry, School of Medicine, Gachon University, Incheon 406-840, Korea

4Gachon Medical Research Institute, Gil Medical Center, Incheon 405-760, Korea

Correspondence to:

Wook Jin, email: jinwo@gachon.ac.kr

Keywords: TrkC, colorectal cancer, tumorigenicity, metastasis, EMT program

Received: August 05, 2016    Accepted: April 03, 2017    Published: April 20, 2017

ABSTRACT

The current work reveals that TrkC receptor is crucial to many aspects of tumorigenicity and metastasis of cancer. However, with only a few exceptions, such as colorectal cancer (CRC), where suppressing tumorigenic and metastatic ability via expression of TrkC as tumor suppressor have been proposed. These diverse lines of evidence led us to investigate whether TrkC is involved in CRC progression. By using mouse models and molecular biology analyses, we demonstrate that TrkC acts as an activator in tumorigenicity and metastasis of colorectal cancer. In this study, TrkC was frequently overexpressed in CRC cells, patients’ tumor samples and an azoxymethane/dextran sulphate sodium-induced mouse model of colitis-associated CRCs. TrkC expression was associated with a high-grade CRC phenotype, leading to significantly poorer survival. Also, TrkC expression promoted the acquisition of motility and invasiveness in CRC. Moreover, TrkC increased the ability to form tumor spheroids, a property associated with cancer stem cells. Importantly, knockdown of TrkC in malignant mouse or human CRC cells inhibited tumor growth and metastasis in a mouse xenograft model. Furthermore, TrkC enhanced metastatic potential and induced proliferation by aberrant gain of AKT activation and suppression of transforming growth factor (TGF)-β signalling. Interestingly, TrkC not only modulated the actions of TGF-β type II receptor, but also attenuated expression of this receptor. These findings reveal an unexpected physiological role of TrkC in the pathogenesis of CRC. Therefore, TrkC is a potential target for designing effective therapeutic strategies for CRC development.


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