Research Papers:

Inhibitory effect of ethanol extract of Nannochloropsis oceanica on lipopolysaccharide-induced neuroinflammation, oxidative stress, amyloidogenesis and memory impairment

Ji Yeon Choi, Chul Ju Hwang, Hee Pom Lee, Hee Sik Kim, Sang-Bae Han and Jin Tae Hong _

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Oncotarget. 2017; 8:45517-45530. https://doi.org/10.18632/oncotarget.17268

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Ji Yeon Choi1, Chul Ju Hwang1, Hee Pom Lee1, Hee Sik Kim2, Sang-Bae Han1 and Jin Tae Hong1

1College of Pharmacy and Medical Research Center, Chungbuk National University, Osong-eup, Heungdeok-gu, Cheongju, Chungbuk 28160, Republic of Korea

2Sustainable Bioresource Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseoung, Daejeon 305-806, Republic of Korea

Correspondence to:

Jin Tae Hong, email: [email protected]

Keywords: neuroinflammation, amyloidogenesis, oxidation, NF-κB, Nannochloropsis oceanica

Received: January 21, 2017     Accepted: March 26, 2017     Published: April 20, 2017


Oxidative stress and neuroinflammation is implicated in the pathogenesis and development of Alzheimer’s disease (AD). Here, we investigated the suppressive possibility of ethanol extract of Nannochloropsis oceanica (N. oceanica) on memory deficiency along with the fundamental mechanisms in lipopolysaccharide (LPS)-treated mice model. Among several extracts of 32 marine microalgae, ethanol extract of N. oceanica showed the most significant inhibitory effect on nitric oxide (NO) generation, NF-κB activity and β-secretase activity in cultured BV-2 cells, neuronal cells and Raw 264.7 cells. Ethanol extract of N. oceanica (50, 100 mg/kg) also ameliorated LPS (250 μg/kg)-induced memory impairment. We also found that ethanol extract of N. oceanica inhibited the LPS-induced expression of iNOS and COX-2. Furthermore, the production of reactive oxygen species (ROS), malondialdehyde (MDA) level as well as glutathione (GSH) level was also decreased by treatment of ethanol extract of N.oceanica. The ethanol extract of N. oceanica also suppresses IκB degradation as well as p50 and p65 translocation into the nucleus in LPS-treated mice brain. Associated with the inhibitory effect on neuroinflammation and oxidative stress, ethanol extract of N. oceanica suppressed Aβ1-42 generation through down-regulation of APP and BACE1 expression in in vivo. These results suggest that ethanol extract of N. oceanica ameliorated memory impairment via anti-inflammatory, anti-oxidant and anti-amyloidogenic mechanisms.

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