Nano-pulse stimulation (NPS) ablate tumors and inhibit lung metastasis on both canine spontaneous osteosarcoma and murine transplanted hepatocellular carcinoma with high metastatic potential
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Xinhua Chen1,*, Yiling Chen2,*, Jianwen Jiang1, Liming Wu1, Shengyong Yin1, Xudong Miao3, Robert J. Swanson4 and Shusen Zheng1
1Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health, Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University, Zhejiang, 310003, China
2The Department of Anatomy, Tianjin Medical University, Tianjin, 300070, China
3The Department of Orthopedics, The Second Affiliated Hospital, Zhejiang University, Zhejiang, 310003, China
4Anatomical Sciences Department, College of Osteopathic Medicine, Liberty University, Lynchburg, VA, 24515, USA
*These authors have contributed equally to this work
Shusen Zheng, email: [email protected]
Xudong Miao, email: [email protected]
Robert J. Swanson, email: [email protected]
Keywords: nano-pulse stimulation (NPS), nanosecond pulsed electric field (nsPEF), non-thermal, ablation, palliative therapy
Abbreviations: nsPEF: nanosecond pulsed electric field; ALP: alkaline phosphatase; NPS: nano-pulse stimulation
Received: December 16, 2016 Accepted: March 26, 2017 Published: April 18, 2017
Background: Nanosecond pulsed electric field (nsPEF), which is also termed as nano-pulse stimulation (NPS), has the potential of stimulating immune responses toward cancer cells. The current study investigates its local and systemic antitumor efficacy in vivo in late stage tumors with lung metastasis.
Method: The 12 canines with spontaneous osteosarcomas and 12 nude mice transplanted with human hepatocellular carcinoma were divided randomly and were given NPS treatment, surgery or no treatment control. Nanosecond pulsed electric field was delivered with puncture electrodes at 40 kV/cm with 500 pulses at 1 Hz. The survival time, tumor volume, serum alkaline phosphatase (ALP), joint capsule damage and lung metastasis were followed up. The efficacy was compared with control.
Results: Nanosecond pulsed electric field reduced primary tumor volume and extended the survival significantly compared to the control group (P<0.05). Inhibition of serum alkaline phosphatase and lung metastasis without joint deformity or thermal damage were also observed.
Conclusion: Locally applied nanosecond pulsed electric field is a novel non-thermal ablation method. It can ablate the primary tumor and decrease lung metastasis as a palliative therapy for late stage tumor.
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