Oncotarget

Research Papers:

LncRNA AFAP1-AS1 promotes growth and metastasis of cholangiocarcinoma cells

Xiuhui Shi, Hang Zhang, Min Wang, Xiaodong Xu, Yan Zhao, Ruizhi He, Min Zhang, Min Zhou, Xu Li, Feng Peng, Chengjian Shi, Ming Shen, Xin Wang, Xingjun Guo and Renyi Qin _

PDF  |  HTML  |  How to cite  |  Order a Reprint

Oncotarget. 2017; 8:58394-58404. https://doi.org/10.18632/oncotarget.16880

Metrics: PDF 3081 views  |   HTML 2001 views  |   ?  


Abstract

Xiuhui Shi1,*, Hang Zhang1,*, Min Wang1,*, Xiaodong Xu1, Yan Zhao1, Ruizhi He1, Min Zhang1, Min Zhou1, Xu Li1, Feng Peng1, Chengjian Shi1, Ming Shen1, Xin Wang1, Xingjun Guo1 and Renyi Qin1

1Department of Biliary-Pancreatic Surgery, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

*These authors have contributed equally to this study

Correspondence to:

Renyi Qin, email: ryqin@tjh.tjmu.edu.cn

Xingjun Guo, email: xjguo@tjh.tjmu.edu.cn

Keywords: long noncoding RNA, AFAP1-AS1, cholangiocarcinoma, proliferation, metastasis

Received: February 07, 2017    Accepted: March 15, 2017    Published: April 06, 2017

ABSTRACT

We investigated the role of actin filament associated protein 1 antisense RNA1 (AFAP1-AS1) lncRNA in promoting cholangiocarcinoma (CCA). qRT-PCR analysis of patient samples showed that AFAP1-AS1 expression was higher in CCA tumors than matched adjacent non-tumor tissue. AFAP1-AS1 levels were also higher in CCA cell lines (HuCCT1 and TFK-1) than a normal biliary epithelium cell line (HIBEpic). AFAP1-AS1 knockdown in CCA cell lines using shAFAP1-AS1 reduced cell proliferation and colony formation in CCK-8 and colony formation assays, respectively. Cell cycle analysis demonstrated that AFAP1-AS1 knockdown resulted in G0/G1 cell cycle arrest and inhibition of S-G2/M transition compared to the controls. CCA cells transfected with shAFAP1-AS1 also exhibited reduced metastasis and invasiveness in Transwell and wound healing assays. This was further confirmed in xenograft experiments with nude mice using CCA cells transfected with shAFAP1-AS1 or control shRNA. AFAP1-AS1 knockdown cells produced smaller tumors, demonstrating that AFAP1-AS1 promotes tumor growth in vivo. AFAP1-AS1 knockdown also increased expression of actin filament associated protein 1 (AFAP1) and reduced cell stress filament integrity, as determined from western blot and immunofluorescence assays, respectively. These findings indicate that AFAP1-AS1 exerts oncogenic effects in CCA. We postulate that AFAP1-AS1 is a potentially useful diagnostic and prognostic biomarker and therapeutic target for CCA.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 16880