Combination analysis of NOS3, ABCB1 and IL23R polymorphisms with alcohol-induced osteonecrosis of the femoral head risk in Chinese males
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Yuan Wang1,*, Xuejun Yang2,3,*, Jianping Shi3, Yan Zhao2,3, Linlin Pan2,3, Jinqiu Zhou4, Guoqiang Wang2,3 and Jianzhong Wang2,3
1Department of Orthopedics, the People’s Hospital of Manzhouli City, Manzhouli 021400, Inner Mongolia, China
2Department of Orthopedics, the Second Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia, China
3The College of Traditional Chinese Medicine, Inner Mongolia Medical University, Hohhot 010010, Inner Mongolia, China
4Department of Endocrine, the 253th Hospital of People’s Liberation Army of China, Hohhot 010010, Inner Mongolia, China
*The first two authors should be regarded as joint first authors
Jianzhong Wang, email: email@example.com
Guoqiang Wang, email: firstname.lastname@example.org
Keywords: NOS3, ABCB1, IL23R, osteonecrosis of the femoral head, gene polymorphism
Received: July 10, 2016 Accepted: March 16, 2017 Published: April 04, 2017
Background: Common variants of multiple genes played a crucial role in osteonecrosis of the femoral head (ONFH) onset which was proved by many previous reports. We hypothesized that polymorphisms in NOS3, ABCB1 and IL23R were related to individual differences in alcohol sensitivity and the development of alcohol-induced ONFH.
Methods: In this case-control study, we evaluated 8 SNPs in three genes in the Chinese Han population including 355 male cases and 355 healthy male controls. These SNPs were genotyped by Sequenom MassARRAY RS1000. To identify their relationship with alcohol-induced ONFH susceptibility using χ2 test and genetic model analysis.
Results: We found an association with alcohol-induced ONFH susceptibility for 4 SNPs (rs743506, rs3918184, rs13233308 and rs6693831) in three genes after adjusted by age. The genotype “G/A” of rs743506 in NOS3 gene acts as a risk factor in genotype (P = 0.003), dominant (P = 0.048), recessive (P = 0.005) and additive model(P = 0.006); The genotype “T/C” of rs3918184 in NOS3 gene acts as a risk factor in genotype (P = 0.012) and recessive model (P = 0.009); The genotype “T/C” of rs13233308 in ABCB1 gene acts as a risk factor in genotype (P = 0.038) and additive model(P = 0.041); The genotype “T/C” of rs6693831 in IL23R gene acts as a protective factor in genotype model (P = 0.046).
Conclusions: This study provides evidence for three alcohol-induced ONFH susceptibility genes (NOS3, ABCB1 and IL23R) in Chinese males and polymorphisms of them may be associated with alcohol-induced ONFH risk.
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