Oncotarget

Research Papers:

Silencing of karyopherin α2 inhibits cell growth and survival in human hepatocellular carcinoma

Yunfeng Yang, Jian Guo, Yuxia Hao, Fuhua Wang, Fengxia Li, Shaomin Shuang and Junping Wang _

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Oncotarget. 2017; 8:36289-36304. https://doi.org/10.18632/oncotarget.16749

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Abstract

Yunfeng Yang1,2, Jian Guo3, Yuxia Hao2, Fuhua Wang4, Fengxia Li2, Shaomin Shuang1, Junping Wang2

1College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, 030006, Shanxi, China

2Department of Gastroenterology, Shanxi Provincial People’s Hospital, Taiyuan, 030012, Shanxi, China

3Department of General Surgery, Shanxi Provincial People’s Hospital, Taiyuan, 030012, Shanxi, China

4Department of Molecular Biology, Shanxi Cancer Hospital and Institute, Taiyuan, 030013, Shanxi, China

Correspondence to:

Shaomin Shuang, email: ssm0958@yahoo.com

Junping Wang, email: wangjp8396@sohu.com

Keywords: hepatocellular carcinoma, KPNA2, tumorigenesis, microarray, gene-interaction network

Received: September 09, 2016     Accepted: March 20, 2017     Published: March 31, 2017

ABSTRACT

Karyopherin α2 (KPNA2), involved in nucleocytoplasmic transport, has been reported to be upregulated in hepatocellular carcinoma and considered as a biomarker for poor prognosis. However, comprehensive studies of KPNA2 functions in hepatocellular carcinogenesis are still lacking. Our study examine the roles and related molecular mechanisms of KPNA2 in hepatocellular carcinoma development. Results show that KPNA2 knockdown inhibited the proliferation and growth of hepatocellular carcinoma cells in vitro and in vivo. KPNA2 knockdown also inhibited colony formation ability, induced cell cycle arrest and cellular apoptosis in two hepatocellular carcinoma cell lines, HepG2 and SMMC-7721. Furthermore, gene expression microarray analysis in HepG2 cells with KPNA2 knockdown revealed that critical signaling pathways involved in cell proliferation and survival were deregulated. In conclusion, this study provided systematic evidence that KPNA2 was an essential factor promoting hepatocellular carcinoma and unraveled potential molecular pathways and networks underlying KPNA2-induced hepatocellular carcinogenesis.


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