Oncotarget

Research Papers:

Heat stress induces intestinal injury through lysosome- and mitochondria-dependent pathway in vivo and in vitro

Gao Yi _, Li Li, Meijuan Luo, Xuan He, Zhimin Zou, Zhengtao Gu and Lei Su

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Oncotarget. 2017; 8:40741-40755. https://doi.org/10.18632/oncotarget.16580

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Abstract

Gao Yi1,2,*, Li Li3,4,*, Meijuan Luo5,*, Xuan He1, Zhimin Zou1, Zhengtao Gu3,4 and Lei Su1,6

1Southern Medical University, Guangzhou, 510515, P.R. China

2The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510700, P.R. China

3Department of Intensive Care Unit, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, P.R. China

4Department of Pathophysiology, Southern Medical University, Guangdong Provincial Key Laboratory of Shock and Microcirculation Research, Guangzhou 510515, P.R. China

5Department of Pediatrics, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou, 510180, P.R. China

6Department of Intensive Care Unit, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, 510010, P.R. China

*These authors have contributed equally to this work

Correspondence to:

Zhengtao Gu, email: guzhengtao@126com

Lei Su, email: sulei_icu@163.com

Keywords: heat stress, cathepsin B, lysosomal membrane permeabilization, mitochondrial, apoptosis

Received: September 18, 2016    Accepted: December 08, 2016    Published: March 28, 2017

ABSTRACT

Damage to the small intestine secondary to heat stroke is a major factor in heat stroke-related morbidity and mortality. However, the underlying mechanisms by which heat stroke causes small intestinal lesions and dysfunction remain unclear. To explore the pathogenesis of small intestinal tissue and epithelial cell injury, the SW480 cell heat stress model and the mice heat stroke model were established to mimic heat stroke. Morphologic changes in intestinal tissue and increased TUNEL-positive index were induced by heat stress in vivo. Heat stress activated the lysosomal-mitochondrial apoptotic pathway in SW480 cells, increasing intracellular reactive oxygen species and causing lysosomal membrane permeabilization with subsequent release of cathepsin B to the cytosol, mitochondrial depolarization, and cytochrome C release to cytosol. An increase in the Bax/Bcl2 ratio, caspase-9 and caspase-3 were observed. N-Acetyl-L-Cysteine was shown to inhibit ROS generation, suppress permeabilization of lysosomal membranes, decrease levels of cathepsin B and cytochrome C in the cytosol, and inhibit Bax/Bcl2 ratio, caspase-9 and caspase-3 activity both in vitro and in vivo. Mitochondrial damage was alleviated when the models were pre-treated with CA-074 Me both in vitro and in vivo, decreasing cathepsin B and cytochrome C levels in the cytosol, Bax/Bcl2 ratio, caspase-9 and caspase-3 activity. In our models, heat stress-induced apoptosis of small intestinal tissue and epithelial cells through accumulation of ROS and activation of the lysosomal–mitochondrial apoptotic pathway involved the release of cathepsin B. These findings may offer potentially pharmaceutical targets and strategies to repair intestinal injury caused by heat stroke.


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