Micro RNA-19a interferes with IL-10 expression in peripheral dendritic cells of patients with nasal polyposis
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Xiang-Qian Luo1,2,*, Jian-Bo Shao1,2,*, Rui-Di Xie2, Lu Zeng2, Xiao-Xi Li2, Shu-Qi Qiu3, Xiao-Rui Geng3, Li-Tao Yang3,4, Lin-Jing Li4, Da-Bo Liu1, Zhi-Gang Liu2 and Ping-Chang Yang2
1Department of Otolaryngology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou 510010, China
2The Research Center of Allergy and Immunology, Shenzhen University School of Medicine, Shenzhen 518060, China
3Longgang ENT Hospital, Shenzhen 518116, China
4Brain Body Institute, McMaster University, Hamilton, ON, L8N 4A6, Canada
*These authors equally contributed to this work
Ping-Chang Yang, email: firstname.lastname@example.org
Zhi-Gang Liu, email: email@example.com
Keywords: nose, nasal polyp, dendritic cells, micro RNA, interleukin-10
Received: November 02, 2016 Accepted: March 14, 2017 Published: March 24, 2017
The pathogenesis of nasal polyp is to be further investigated. Micro RNA (miR) plays a role in the development of allergic inflammation. Interleukin (IL)-10-producing dendritic cells (DC) have immune tolerogenic properties. This study test a hypothesis that miR-17-92 cluster is associated with suppressing IL-10 in peripheral DC. In this study, peripheral blood samples were obtained from 26 patients with nasal polyp. The CD11c DCs were isolated from the blood samples and analyzed for the expression of IL-10. We observed that, as compared with healthy subjects, the IL-10 expression in peripheral DC was significantly lower in polyp patients. The levels of miR-19a, but not the rest 5 members of the miR-17-92 cluster, were markedly higher in DCs in polyp group. Exposure to recombinant IL-4 suppressed the IL-10 expression in DCs, which was abolished by blocking histone deacetylase-11 or knocking down the miR-19a gene in DCs. We conclude that miR-19a plays a critical role in the suppression of IL-10 in peripheral DCs, which may be a target in the immune therapy for nasal polyp.
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